SODD/BAG4 Antibody [Alexa Fluor® 350]

• Reactivity: Mu, Rt
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

SODD/BAG4 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant mouse SODD/BAG4
  Met1-Leu457
  Accession # Q8CI61
• Specificity: Detects endogenous mouse and rat SODD/BAG4 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SODD/BAG4 Antibody [Alexa Fluor® 350]

• BAG4
• BAG-4
• BAG-family molecular chaperone regulator-4
• Bcl-2-associated athanogene 4
• BCL2-associated athanogene 4
• Silencer of death domains
• SODD
• SODDBAG family molecular chaperone regulator 4

Background

Tumor necrosis factor receptor-1 (TNF-R1) and other TNF receptor superfamily members, such as DR3, contain intracellular death domains (DD) and are capable of initiating apoptosis when activated by their ligands. Silencer of Death Domains (SODD) was identified as being involved in the cellular mechanism to protect against ligand-independent signaling by TNF-R1 and other DD receptors. SODD, also known as Bcl-2-Associated Athanogene 4 (BAG4), is a 457 amino acid (aa), anti‑apoptotic protein that functions through interactions with a variety of proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors, and members of the heat shock protein 70 kDa family. SODD is a ubiquitously expressed, cytoplasmic protein that contains a C terminal BAG domain that can bind and inhibit the chaperone activity of Hsc70/Hsp70. The association of SODD with the DD of TNF-R1 prevents constitutive activation of the TNF-R1 signaling pathway. Binding of TNF to TNF-R1 releases SODD and permits adapter molecules such as TRADD to associate with TNF-R1 leading to the activation of TNF signaling pathways such as apoptosis and NF kappa B activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.