SOCS-2 Antibody (JG36-86)

Western Blot: SOCS-2 Antibody (JG36-86) [NBP2-75671] - Analysis of SOCS-2 on K562 cell lysate lysates with Rabbit anti-SOCS-2 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: SOCS-2 Antibody (JG36-86) [NBP2-75671] - Analysis of LOVO cells labeling SOCS2 with Rabbit anti-SOCS-2 antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOCS-2 antibody. Nuclear DNA was labelled in blue with DAPI.

Western Blot: SOCS-2 Antibody (JG36-86) [NBP2-75671] - Analysis of SOCS2 on different lysates using anti-SOCS2 antibody at 1/1,000 dilution.Positive control:Lane 1: Human placenta Lane 2: SH-SY-5Y

Immunocytochemistry/Immunofluorescence: SOCS-2 Antibody (JG36-86) [NBP2-75671] - Staining SOCS2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SOCS-2 Antibody (JG36-86) [NBP2-75671] - Staining SOCS2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SOCS-2 Antibody (JG36-86) [NBP2-75671] - Staining SOCS2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

SOCS-2 Antibody (JG36-86) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SOCS-2 aa 1-198 / 198. (SwissProt: O14508 Human)
• Localization: Cytosol.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SOCS2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SOCS-2 Antibody (JG36-86)

• CIS2
• CIS2CIS-2
• Cish2
• Cytokine-inducible SH2 protein 2
• SOCS2
• SOCS-2
• SOCS-2STAT-induced STAT inhibitor 2
• SSI2
• SSI-2STAT induced STAT inhibitor-2
• SSI2STAT-induced STAT inhibitor-2
• STATI2
• STATI2suppressor of cytokine signaling-2
• suppressor of cytokine signaling 2

Background

Accumulating evidence demonstrates that cytokine receptorsignaling is negatively regulated by a family of Srchomology 2 domain-containing adaptor molecules TermedSOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have beenrecognized, they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an Nterminalregion of variable length and amino acidcomposition, a central SH2 domain, and a previouslyunrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of aclassical negative feed back loop that regulates cytokinesignal transduction via a STAT induced transcriptionalmechanism. Transcription of each of the SOCS genesoccurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCSfamily appear to inhibit signaling in different ways. SOCS 2, not like SOCS 1 and SOCS 3, regulates postnatalgrowth, likely through its ability to influence the GH / IGF 1axis, although they might have overlapping actions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOCS2