SMPD3/NSMase2 Antibody (758612) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB
• Clone: 758612
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

SMPD3/NSMase2 Antibody (758612) [Alexa Fluor® 594] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human SMPD3
  Val2-Ala655
  Accession # Q9NY59
• Specificity: Detects human SMPD3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SMPD1 or rhPP2A is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SMPD3/NSMase2 Antibody (758612) [Alexa Fluor® 594]

• EC 3.1.4.12
• FLJ22593
• MGC138443
• Neutral sphingomyelinase 2
• Neutral sphingomyelinase II
• NSMASE2
• nSMase-2
• SMPD3
• sphingomyelin phosphodiesterase 3
• sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinaseII)

Background

SMPD3 (Sphingomyelin phosphodiesterase 3; also Neutral sphingomyelinase 2/nSMase2) is a 69-74 kDa member of the neutral sphingomyelinase family of enzymes. It is a monomeric Golgi/plasma membrane enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide and phosphorylcholine. This generates second messenger components that participate in signal transduction. Human SMPD3 is a two transmembrane, 655 amino acids molecule. It contains an N-terminal luminal segment (aa 1-10), a cytoplasmic region (aa 32-64), and one catalytic domain (aa 340-646). Phosphorylation increases its MW to 78 kDa in SDS-PAGE. There is one potential isoform that possesses an Asn substitution for aa 569-587. Over aa 2-655, human SMPD3 shares 91% aa sequence identity with mouse SMPD3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.