SMPD1 Antibody

Western Blot: SMPD1 Antibody [NBP2-20426] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-20426] - Human breast cancer, using ASM antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Discontinued

SMPD1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SMPD1. The exact sequence is proprietary.
• Localization: Lysosome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMPD1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (85%), Rat (86%), Sheep (85%), Bovine (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SMPD1 Antibody

• Acid sphingomyelinase
• aSMase
• ASMsphingomyelin phosphodiesterase
• EC 3.1.4.12
• NPD
• SMPD1
• sphingomyelin phosphodiesterase 1, acid lysosomal

Background

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SMPD1
• Uniprot:
  • P17405(Human)