SMPD1 Antibody [Alexa Fluor™ Plus 680]

• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 680

SMPD1 Antibody [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects human SMPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SMPD1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Sphingomyelin phosphodiesterase, also known as acid sphingomyelinase and encoded by the SMPD1 gene, is a lysosomal phosphodiesterase which belongs to the acid sphingomyelinase family (1). SMPD1 catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. Ceramide, a bioactive lipid, has emerged as an important signaling molecule involved in a variety of cellular processes such as cell differentiation, apoptosis, and proliferation (2). Activation of SMPD1 occurs by the removal, chemical modification or dimerization of its C-terminal cysteine residue (3). Deficiencies of SMPD1 result in a lysosomal storage disorder referred to as Niemann-Pick disease (4). rhSMPD1 was expressed without the last three C-terminal residues, and is therefore constitutively active.

1. Schuchman, E.H. et al. (1991) J. Biol. Chem. 266:8531.
2. Melendez, A.J. et al. (2008) Biochim. Biophys. Acta 1784:66.
3. Qiu, H. et al. (2003) J. Biol. Chem. 278:32744.
4. Smith, E.L. and Schuchman, E.H. (2008) FASEB J. 22:3419.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.