Smoothened Luciferase - (LUCPorter™) Stable Reporter Cell Line

Product Discontinued

Smoothened Luciferase - (LUCPorter™) Stable Reporter Cell Line Summary

• Description: The SMO/LUCPoter(TM) reporter cell line is designed to monitor the induction of SMO and can be used for screening of agonists, antagonists or signaling inhibitors of SMO induction as well as for studying the SMO induction-related signaling pathways.
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: The SMO/LUCPorter(TM) reporter cell line is a stably transfected HEK 293 cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the Smoothened (SMO) promoter. As a G protein-coupled receptor, SMO is a key transducer of the Hedgehog signaling pathway, which plays a critical role in tissue maintenance and repair. Recent studies have demonstrated that the Hedgehog pathway is a key element in the development of many forms of cancer, in which SMO plays an essential role. Therefore the therapeutic value of SMO inhibitors/antagonists for treating Hedgehog-related cancers has been the subject of intense academic and clinical research. The SMO/LUCPorter(TM) reporter cell line, which can be used as a tool for identification of SMO induction inhibitors, is an inducible reporter cell line. This cell line can be induced by treatment of phorbol 12-myristate 13-acetate (PMA) as shown in Figure 1.
• Selection Agent: Puromycin at 3 ug/ml
• Growth Properties: Adherent
  Morphology : Epithelial
• RCL Type: Luciferase - (LUCPorter™)
• Host: HEK293
• Gene: SMO

Applications/Dilutions

• Dilutions:
  • Ligand Activation
• Readout System:
  • LightSwitch Luciferase Assay Reagent NBP2-32066

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin (Note: The selection agent for this cell line is puromycin at 3 ug/ml).

Alternate Names for Smoothened Luciferase - (LUCPorter™) Stable Reporter Cell Line

• bnb
• CRJS
• FZD11
• Gx
• Protein Gx
• SMO
• SMOH
• SMOHseven transmembrane helix receptor
• smoothened (Drosophila) homolog
• smoothened homolog (Drosophila)
• smoothened homolog
• Smoothened

Background

Smoothened, a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas. Smoothened expression has been documented in bladder, blood, brain, the central nervous system, skin, and ureter. ESTs have been isolated from amnion, bone, brain, breast, embryo, eye, heart, nerve, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMO