Smoothened Antibody

Product Discontinued

Smoothened Antibody Summary

• Immunogen: Peptide with sequence C-QSDDEPKRIKKS corresponding to internal region according to NP_005622.1.
• Epitope: QSDDEPKRIKKS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SMO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human bone marrow lysates (calculated MW of 86.4 kDa band according to NP_005622.1). The observed molecular weight corresponds to earlier findings with NB100-55392, a product of different design reacting to a different part of the same protein.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Zebrafish.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Smoothened Antibody

• bnb
• CRJS
• FZD11
• Gx
• Protein Gx
• SMO
• SMOH
• SMOHseven transmembrane helix receptor
• smoothened (Drosophila) homolog
• smoothened homolog (Drosophila)
• smoothened homolog
• Smoothened

Background

Smoothened (SMOH), a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein (PTCH) to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas. SMO expression has been documented in bladder, blood, brain, the central nervous system, skin, and ureter. ESTs have been isolated from amnion, bone, brain, breast, embryo, eye, heart, nerve, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smoothened Antibody (NB100-55397)(1)

Publication using NB100-55397

• Taipale J, Cooper MK, Maiti T, Beachy PA. Patched acts catalytically to suppress the activity of Smoothened. Nature 2002-08-22 [PMID: 12192414]

Bioinformatics

• Gene Symbol: SMO
• Entrez:
  • 6608(Human)
  • 319757(Mouse)
  • 25273(Rat)
• Uniprot:
  • NP_005622.1(Human)