SMOC-2 Antibody [Unconjugated] - Isoform 1

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SMOC-2 Antibody [Unconjugated] - Isoform 1 Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SMOC-2
  Gln22-Gly446
  Accession # Q9H3U7
• Specificity: Detects human and mouse SMOC‑2 in direct ELISAs and Western Blots
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SMOC2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SMOC-2 Antibody [Unconjugated] - Isoform 1

• bA270C4A.1
• bA37D8.1
• dJ421D16.1
• MST117
• MSTP117
• MSTP140
• SMAP2
• SMAP-2
• SMAP2Secreted modular calcium-binding protein 2
• SMOC2
• SMOC-2
• smooth muscle associated protein 2
• Smooth muscle-associated protein 2
• SPARC related modular calcium binding 2
• SPARC-related modular calcium-binding protein 2
• thyroglobulin type-1 repeat containing protein

Background

SMOC-2 (secreted modular calcium-binding protein 2; also SMAP2) is a 55 kDa glycoprotein member of the SPARC family of matricellular proteins. It is widely expressed, perhaps by endothelium and apparently participates in both growth factor signaling and ECM organization. Mature human SMOC-2 is 425 amino acids (aa) in length. It contains one Kazal-like domain (aa 40‑84), two thyroglobulin type-1 segments (aa 87‑153 and 213‑281) and two EF-hand sequences (aa 347‑382 and 384‑419). There are three splice variants. One shows a 13 aa substitution for aa 442‑446, another shows an 11 aa insertion after Thr170 and a third represents a combination of the above two variations. Over aa 22‑446, human SMOC-2 shares 95% aa identity with mouse SMOC-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMOC-2 Antibody (AF5140)(2)

Publications using AF5140

• D Feng, C Ngov, N Henley, N Boufaied, C Gerarduzzi Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury Sci Rep, 2019-11-13;9(1):16736. 2019-11-13 [PMID: 31723159] (Western Blot, Mouse)
• C Gerarduzzi, RK Kumar, P Trivedi, AK Ajay, A Iyer, S Boswell, JN Hutchinson, SS Waikar, VS Vaidya Silencing SMOC2 ameliorates kidney fibrosis by inhibiting fibroblast to myofibroblast transformation JCI Insight, 2017-04-20;2(8):. 2017-04-20 [PMID: 28422762] (Functional Assay, Mouse)

Bioinformatics

• Gene Symbol: SMOC2
• Uniprot:
  • Q9H3U7()