SMG7 Antibody - Azide and BSA Free

Western Blot: SMG7 Antibody [NBP2-94721] - Analysis of extracts of various cell lines, using SMG7 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SMG7 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit SMG7 Antibody - Azide and BSA Free (NBP2-94721) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMG7 (NP_963863.2). MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMG7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:200-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for SMG7 Antibody - Azide and BSA Free

• breast cancer-associated antigen SGA-56M
• C1orf16
• chromosome 1 open reading frame 16
• EST1 telomerase component homolog C
• EST1CKIAA0250SGA56M
• EST1-like protein C
• ever shorter telomeres 1C
• FLJ23717
• hSMG-7
• protein SMG7
• SMG-7 homolog
• Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
• SMG-7

Background

SMG7 plays a role in the dephosphorylation of UPF1, an RNA-dependent ATPase and helicase critical to nonsense-mediated mRNA decay (NMD). It is a homolog of the C. elegans NMD factor Smg7 (suppressor with morphogenetic effect on genitalia protein 7). SMG7 functions as an adaptor to target protein phosphatase 2A to UPF1 for dephosphorylation of UPF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: SMG7