Smek1 Antibody

Western Blot: Smek1 Antibody [NB100-68163] - (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Smek1 Antibody Summary

• Description: Novus Biologicals Goat Smek1 Antibody (NB100-68163) is a polyclonal antibody validated for use in WB and ELISA. Anti-Smek1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EQQRERQDNPKLD corresponding to internal region according to NP_115949.3.
• Epitope: EQQRERQDNPKLD
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPP4R3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 110 kDa band observed in human pancreas lysates (calculated MW of 93.9 kDa band according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  Smek1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Smek1 Antibody

• FLFL1
• FLJ20707
• KIAA2010MSTP033
• PP4R3A
• PPP4R3A
• serine/threonine-protein phosphatase 4 regulatory subunit 3A
• SMEK homolog 1
• SMEK homolog 1, suppressor of mek1 (Dictyostelium)
• smk1
• smk-1

Background

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smek1 Antibody (NB100-68163)(1)

Publication using NB100-68163

• Panowski SH, Wolff S, Aguilaniu H et al. PHA-4/Foxa mediates diet-restriction-induced longevity of C. elegans. Nature 2007-05-31 [PMID: 17476212]

Bioinformatics

• Gene Symbol: PPP4R3A
• Entrez:
  • 55671(Human)
  • 68734(Mouse)
  • 314388(Rat)
• Uniprot:
  • NP_115949.3(Human)