SMC3 Antibody (JM10-75)

Western Blot: SMC3 Antibody (JM10-75) [NBP2-67153] - Analysis of SMC3 on different cells lysates using anti-SMC3 antibody at 1/1,000 dilution. Positive control: Line1: HepG2 Line2: NIH-3T3 Line3:PC12

Immunocytochemistry/Immunofluorescence: SMC3 Antibody (JM10-75) [NBP2-67153] - Staining SMC3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SMC3 Antibody (JM10-75) [NBP2-67153] - Analysis of paraffin-embedded zebrafish tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

Flow Cytometry: SMC3 Antibody (JM10-75) [NBP2-67153] - Analysis of Hela cells with SMC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: SMC3 Antibody (JM10-75) [NBP2-67153] - Staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SMC3 Antibody (JM10-75) [NBP2-67153] - Staining SMC3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SMC3 Antibody (JM10-75) [NBP2-67153] - Analysis of paraffin-embedded human colon cancer tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SMC3 Antibody (JM10-75) [NBP2-67153] - Analysis of paraffin-embedded human tonsil tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SMC3 Antibody (JM10-75) [NBP2-67153] - Analysis of paraffin-embedded mouse colon tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

Product Discontinued

SMC3 Antibody (JM10-75) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SMC3 aa 1994-1217 / 1217. (SwissProt: Q9UQE7 Human; SwissProt: Q9CW03 Mouse; SwissProt: P97690 Rat)
• Localization: Centromere, Chromosome, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMC3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SMC3 Antibody (JM10-75)

• Bamacan
• BAMSMC protein 3
• Basement membrane-associated chondroitin proteoglycan
• BMH
• chondroitin sulfate proteoglycan 6 (bamacan)
• Chondroitin sulfate proteoglycan 6
• Chromosome-associated polypeptide
• CSPG6structural maintenance of chromosomes protein 3
• hCAP
• HCAPbamacan
• SMC-3
• SMC3L1CDLS3
• structural maintenance of chromosomes 3

Background

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis and in chromosome movement.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMC3