SMARCA5/SNF2H Antibody - BSA Free

Immunoprecipitation: SMARCA5/SNF2H Antibody [NB100-55311] - Detection of Human SNF2h/ISWI by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SNF2h/ISWI antibody NB100-55311 used for IP at 3 mcg/mg lysate. SNF2h/ISWI was also immunoprecipitated by another rabbit anti-SNF2h/ISWI antibody and NB100-55310, which recognize upstream epitopes. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SMARCA5/SNF2H Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SMARCA5/SNF2H Antibody - BSA Free (NB100-55311) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1002 and the C-terminus (residue 1052) of human SNF2h/ISWI using the numbering given in entry NP_003592.2 (GeneID 8467).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMARCA5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
• Theoretical MW: 122 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SMARCA5/SNF2H Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMARCA5/SNF2H Antibody - BSA Free

• EC 3.6.1
• EC 3.6.4.-
• hISWI
• hSNF2HSWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5
• ISWI
• SMARCA5
• SNF2H
• SNF2Hsucrose nonfermenting-like 5
• subfamily a, member 5
• Sucrose nonfermenting protein 2 homolog
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 5
• WCRF135

Background

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SNF2h/ISWI, also known as WCRF135, is the gene product of SMARCA5 that associates with WSTF (Williams Syndrome transcription factor) and several other nuclear proteins to mediate chromatin remodeling. SNF2h is also part of the NoRC (nucleolar remodeling complex).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMARCA5
• Entrez:
  • 8467(Human)
• Uniprot:
  • O60264(Human)