![ELISA: Human Smad2 ELISA Kit (Colorimetric) [NBP2-76620] - Standard Curve Reference](http://images.novusbio.com/fullsize2/Human-Smad2-ELISA-Kit-(Colorimetric)-ELISA-NBP2-76620-img0001.jpg "ELISA: Human Smad2 ELISA Kit (Colorimetric) [NBP2-76620] - Standard Curve Reference")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma and other biological fluids |
| Standard Curve Range | 78.125 - 5000pg/mL |
| Sensitivity | 46.875pg/mL |
| Specificity | This kit recognizes Human Smad2/MADH2 in samples. No significant cross-reactivity or interference between Human Smad2/MADH2 and analogues was observed. |
| Standard Curve Range | 78.125 - 5000pg/mL |
| Sensitivity | 46.875pg/mL |
| Assay Type | Sandwich-ELISA |
| Inter-Assay | CV% < 5 |
| Intra-Assay | CV% < 4.74 |
| Spike Recovery | 88-111% |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | SMAD2 |
| Storage | Storage is content dependent. |
| Components |
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![EphB2 Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "EphB2 Antibody [Unconjugated]")
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| Gene Symbol | SMAD2 |
![Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 Kda. 1:1000 dilution, 1 hour blocking in milk 5%. Image from a verified customer review.](http://images.novusbio.com/images2/Smad3-Antibody-Western-Blot-NB100-56479-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Smad3 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Smad3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56479-img0006.jpg)
![Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/ml.](http://images.novusbio.com/images2/Smad3-Antibody-Immunohistochemistry-Paraffin-NB100-56479-img0004.jpg)
![Western Blot: SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/ml.](http://images.novusbio.com/images2/SMAD4 Antibody-Western Blot-NBP2-24951-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Smad4 Antibody [NBP2-24951] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SMAD4 Antibody NBP2-24951 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/images2/Smad4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24951-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of SMAD4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Apical cytoplasmic staining was observed in both glandular and epithelial cells.](http://images.novusbio.com/images2/Smad4-Antibody-Immunohistochemistry-Paraffin-NBP2-24951-img0002.jpg)
![Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK2 monoclonal antibody.](http://images.novusbio.com/images2/ERK2-Antibody-(6E5)-Western-Blot-NBP1-47842-img0011.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Endometrium tissue within the normal limits using antiMAPK1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,Dilution 1:50)](http://images.novusbio.com/images2/ERK2-Antibody-(6E5)-Immunohistochemistry-Paraffin-NBP1-47842-img0019.jpg)
![Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERK2.](http://images.novusbio.com/images2/ERK2-Antibody-(6E5)-Western-Blot-NBP1-47842-img0010.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Western-Blot-NBP2-22203-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Western Blot: SMAD1/5/8/9 Antibody [NB100-56443] - Lysate fromA549 cells probed with SMAD antibody at 2 ug/mL.](http://images.novusbio.com/images2/SMAD1-5-8-9 Antibody-Western Blot-NB100-56443-img0003.jpg)
![Western Blot: GARS Antibody [NBP1-85533] - Analysis using Anti-GARS antibody NBP1-85533 (A) shows similar pattern to independent antibody NBP1-85534 (B).](http://images.novusbio.com/images2/GARS-Antibody-Western-Blot-NBP1-85533-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GARS Antibody [NBP1-85533] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/GARS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85533-img0006.jpg)
![Immunohistochemistry-Paraffin: GARS Antibody [NBP1-85533] - Staining of human liver.](http://images.novusbio.com/images2/GARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85533-img0011.jpg)
![Western Blot: Fibronectin Antibody [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (http://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Fibronectin-Antibody-Western-Blot-NBP1-91258-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0012.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunohistochemistry-Frozen-NBP1-91258-img0019.jpg)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).](http://images.novusbio.com/images2/p21-CIP1-CDKN1A-Antibody-(WA-1-(HJ21))-Immunohistochemistry-Paraffin-NBP2-29463-img0001.jpg)
![Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Extracts from Jurkat cells.](http://images.novusbio.com/images2/Serpin-E1-PAI-1-Antibody-Western-Blot-NBP1-19773-img0005.jpg)
![Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP1-19773] - Staining of PAI1/Serpine 1 in mouse pancreas.](http://images.novusbio.com/images2/Serpin-E1-PAI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-19773-img0007.jpg)
![Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Immunoblots showing protein expression of ALK5, pSMAD2/3, PAI-1, pVHL, andI2-actin in A498 cells after treatment with TGF-i2 at given point of time. Image collected and cropped by Cite Ab from the following publication (VHL status regulates transforming growth factor B signaling pathways in renal cell carcinoma. Oncotarget (2018)) licensed under a CC-BY licence.](http://images.novusbio.com/images2/Serpin-E1-PAI-1-Antibody-Western-Blot-NBP1-19773-img0008.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Western-Blot-NBP2-37576-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunohistochemistry-NBP2-37576-img0007.jpg)