Smad2 [p Ser465, p Ser467] Antibody

Product Discontinued

Smad2 [p Ser465, p Ser467] Antibody Summary

• Immunogen: KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2 (NM_001003652.2).
• Modification: p Ser465, p Ser467
• Localization: Cytoplasmic and Nuclear
• Specificity: By western blot, the recognizes a band migrating at approximately 58 kDa corresponding to Smad2 only when dually phosphorylated at Ser465 and Ser467. The may also detect phosphorylated Smad3 (Ser423 and Ser425). This antibody does not cross-react with other Smad-related proteins.
• Predicted Species: Mouse (100%), Rat (100%), Chicken (100%), Xenopus (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMAD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Knockdown Validated
  • Western Blot 1:1000
• Application Notes: Western blotting: For best results, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 Use in KD reported in secitific publication PMID: 32547319
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH 7.4) and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Smad2 [p Ser465, p Ser467] Antibody

• hMAD-2
• hSMAD2
• JV18
• JV18-1
• JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
• MAD homolog 2
• Mad protein homolog
• Mad, mothers against decapentaplegic homolog 2
• MADH2
• MADH2MGC34440
• MADR2
• MADR2MGC22139
• Mad-related protein 2
• mother against DPP homolog 2
• mothers against decapentaplegic homolog 2
• Mothers against DPP homolog 2
• Sma- and Mad-related protein 2
• SMAD 2
• SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
• SMAD, mothers against DPP homolog 2
• Smad2
• Smox

Background

Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N-terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smads regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of the two TGF beta dependent serines 465 and 467 in the C terminus of Smad2 provide a recognition site for interaction with Smad4. The phosphorylation of Smad2 on these two serine sites is critical for Smad2 transcriptional activity and TGF beta signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad2 Antibody (NB200-629)(6)

Publications using NB200-629

• Han Y, Yoon Y, Go G et al. Melatonin Protects Human Renal Proximal Tubule Epithelial Cells Against High Glucose-Mediated Fibrosis via the Cellular Prion Protein-TGF-beta-Smad Signaling Axis Int. J. Med. Sci. 2020-05-24 [PMID: 32547319] (KD, WB, Human)
• Yong-SeokHan, Jun HeeLee, Sang Hun Lee Melatonin suppresses ischemia-induced fibrosis by regulating miR-149 Biochem. Biophys. Res. Commun. 2020-02-21 [PMID: 32089265] (WB, Mouse)
• Kim Kyung-Hyun, Sung Hyun-Jung, Lee Woo-Ram et al. Effects of Melittin Treatment in Cholangitis and Biliary Fibrosis in a Model of Xenobiotic-Induced Cholestasis in Mice. Toxins 2015-01-01 [PMID: 26308055] (WB, Mouse)
• Samanta D, Gilkes D, Chaturvedi P, Xiang L, Semenza G. Hypoxia-inducible factors are required for chemotherapy resistance of breast cancer stem cells. Proc Natl Acad Sci U S A. 2014-12-01 [PMID: 25453096] (WB, Human)
• Panno ML, Giordano F, Rizza P et al. Bergapten induces ER depletion in breast cancer cells through SMAD4-mediated ubiquitination Breast Cancer Res Treat 2012-11-01 [PMID: 23053665] (WB, Human)
• Yamamura S, Matsumura N, Mandai M et al. The activated transforming growth factor-beta signaling pathway in peritoneal metastases is a potentialtherapeutic target in ovarian cancer. Int J Cancer 2012-01-01 [PMID: 21503873] (IHC-P, Human)

Review for Smad2 Antibody (NB200-629) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 06/04/2015

Summary

• Application: Western Blot
• Species: Human

Bioinformatics

• Gene Symbol: SMAD2
• Entrez:
  • 4087(Human)
  • 17126(Mouse)
• Uniprot:
  • Q15796(Human)