Smad2/3 Antibody

Images

 
Western Blot: Smad2/3 Antibody [NBP1-19520] - Extracts from PC12 cells.
Immunohistochemistry: Smad2/3 Antibody [NBP1-19520] - Human colon carcinoma tissue.
Western Blot: Smad2/3 Antibody [NBP1-19520] - extracts from COS7 cells.
Western Blot: Smad2/3 Antibody [NBP1-19520] - Western blot (WB) analysis of Smad2/3 (S2) pAb in extracts from PC12 cells.

Product Details

Summary
Product Discontinued
View other related Smad2/3 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-19520
    • Availability
      Product Discontinued

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Smad2/3 Antibody Summary

Immunogen
Synthetic peptide, corresponding to the N-terminal of Human Smad2.
Specificity
This antibody recognizes SMAD2 (Gene ID: 4087, UniProt: Q15796 ) and SMAD3 (Gene ID: 4088, UniProt: P84022).
Clonality
Polyclonal
Host
Rabbit
Gene
SMAD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Smad2/3 (S2) pAb in extracts from PC12 cells.
Immunohistochemistry (IHC) analyzes of Smad2/3 (S2) pAb in paraffin-embedded human colon carcinoma tissue.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-19520 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Smad2/3 Antibody

  • hMAD-2
  • hSMAD2
  • JV18
  • JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
  • MAD homolog 2
  • Mad protein homolog
  • Mad, mothers against decapentaplegic homolog 2
  • MADH2MGC34440
  • MADR2MGC22139
  • Mad-related protein 2
  • mother against DPP homolog 2
  • mothers against decapentaplegic homolog 2
  • Mothers against DPP homolog 2
  • Sma- and Mad-related protein 2
  • SMAD 2
  • SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
  • SMAD, mothers against DPP homolog 2
  • Smad2
  • Smad2/3

Background

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smad2/3 Antibody (NBP1-19520)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Mouse
(1)
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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Smad2/3 NBP1-19520

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Bioinformatics

Gene Symbol SMAD2