Smad2/3 Antibody

Product Discontinued

Smad2/3 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to the N-terminal of Human Smad2.
• Specificity: This antibody recognizes SMAD2 (Gene ID: 4087, UniProt: Q15796 ) and SMAD3 (Gene ID: 4088, UniProt: P84022).
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMAD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Smad2/3 (S2) pAb in extracts from PC12 cells.
  Immunohistochemistry (IHC) analyzes of Smad2/3 (S2) pAb in paraffin-embedded human colon carcinoma tissue.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Smad2/3 Antibody

• hMAD-2
• hSMAD2
• JV18
• JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
• MAD homolog 2
• Mad protein homolog
• Mad, mothers against decapentaplegic homolog 2
• MADH2MGC34440
• MADR2MGC22139
• Mad-related protein 2
• mother against DPP homolog 2
• mothers against decapentaplegic homolog 2
• Mothers against DPP homolog 2
• Sma- and Mad-related protein 2
• SMAD 2
• SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
• SMAD, mothers against DPP homolog 2
• Smad2
• Smad2/3

Background

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad2/3 Antibody (NBP1-19520)(1)

Publication using NBP1-19520

• He XY, Sun K, Xu RS et al. Spatial signalling mediated by the transforming growth factor-b signalling pathway during tooth formation. Int J Oral Sci. 2016-12-16 [PMID: 27982023] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: SMAD2