SLK Knockout 293T Cell Lysate

Product Discontinued

SLK Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,1 bp insertion in exon 5
• Gene: SLK

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for SLK Knockout 293T Cell Lysate

• bA16H23.1
• CTCL tumor antigen se20-9
• EC 2.7.11
• EC 2.7.11.1
• hSLK
• KIAA0204LOSK
• Long Ste20-like Kinase
• se20-9
• Serine/threonine-protein kinase 2
• SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrosenonfermenting like kinase (yeast)
• SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrosenonfermenting like kinase
• SNF1 sucrose nonfermenting like kinase
• STE20-like kinaseSTE20-like kinase (yeast)
• STE20-like serine/threonine-protein kinase
• STE20-related kinase
• Ste20-related serine/threonine kinase
• STE20-related serine/threonine-protein kinase
• STK2MGC133067

Background

The product of this gene belongs to a subfamily of calcium binding proteins, which share similarity to calmodulin. Calcium binding proteins are an important component of calcium mediated cellular signal transduction. Expression of this gene was only detected in retina and brain. Study of the mouse homolog demonstrated that groups of cells expressing this protein are located in the center or inner border of the inner unclear layer of retina. Three alternatively spliced variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SLK