SLC25A22 Antibody - BSA Free

Western Blot: SLC25A22 Antibody [NBP1-33385] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: SLC25A22 Antibody [NBP1-33385] - SLC25A22 antibody detects SLC25A22 protein at mitochondria in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. SLC25A22 antibody diluted at 1:500.

Western Blot: SLC25A22 Antibody [NBP1-33385] - (20 ug of whole cell lysate) mouse brain 10% SDS PAGE; antibody diluted at 1:10000

Immunohistochemistry-Paraffin: SLC25A22 Antibody [NBP1-33385] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:500 dilution.

Immunocytochemistry/ Immunofluorescence: SLC25A22 Antibody [NBP1-33385] - SLC25A22 antibody [N1C3] detects SLC25A22 protein at mitochondria by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SLC25A22 protein stained by SLC25A22 antibody [N1C3] (NBP1-33385) diluted at 1:200.Blue: Hoechst 33342 staining.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SLC25A22 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SLC25A22 Antibody - BSA Free (NBP1-33385) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SLC25A22. The exact sequence is proprietary.
• Localization: Mitochondrion inner membrane, Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC25A22
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SLC25A22 Antibody - BSA Free

• EIEE3mitochondrial glutamate carrier 1
• FLJ13044
• GC1GC-1
• Glutamate/H(+) symporter 1
• NET44
• solute carrier family 25 (mitochondrial carrier: glutamate), member 22
• Solute carrier family 25 member 22

Background

The SLC25 gene family encodes mitochondrial carriers that transport a variety of metabolites across the inner mitochondrial membrane (Palmieri, 2004 [PubMed 14598172]). SLC25A22, also known as GC1, is 1 of the 2 mitochondrial glutamate/H+ symporters, the other being SLC25A18 (MIM 609303).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SLC25A22