Signal sequence receptor delta Antibody (2D3) - Azide and BSA Free

Western Blot: Signal sequence receptor delta Antibody (2D3) [H00006748-M01] - SSR4 monoclonal antibody (M01), clone 2D3 Analysis of SSR4 expression in C32.

Immunocytochemistry/Immunofluorescence: Signal sequence receptor delta Antibody (2D3) [H00006748-M01] - Analysis of monoclonal antibody to SSR4 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: Signal sequence receptor delta Antibody (2D3) [H00006748-M01] - Analysis of SSR4 expression in transfected 293T cell line by SSR4 monoclonal antibody (M01), clone 2D3.Lane 1: SSR4 transfected lysate(19 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: Signal sequence receptor delta Antibody (2D3) [H00006748-M01] - Detection limit for recombinant GST tagged SSR4 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Signal sequence receptor delta Antibody (2D3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SSR4 (AAH03371, 24 a.a. ~ 173 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EACLEPQITPSYYTTSDAVISTETVLIVEISLTCKNRVQNMALYADVGGKQFPVTRGQDVGRYQVSWSLDHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDISIIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGLVIYYLAFSAKSHIQA
• Localization: Endoplasmic reticulum
• Specificity: SSR4 - signal sequence receptor, delta (translocon-associated protein delta)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SSR4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Signal sequence receptor delta Antibody (2D3) - Azide and BSA Free

• signal sequence receptor, delta (translocon-associated protein delta)
• SSR-delta
• translocon-associated protein delta
• translocon-associated protein subunit delta
• TRAP-delta
• TRAPDSignal sequence receptor subunit delta

Background

SSR4, also called TRAPD, is assumed to be involved in protein secretion. It is located in the Xq28 region, arranged in a compact head-to-head manner with the IDH3G gene. These two genes are driven by a bidirectional promoter located between them, and encode proteins involved in unrelated biochemical pathways located in different compartments of the cell. The nontranscribed intergenic region represents only 133 bp and is embedded in a CpG island. The CpG island functions as a bidirectional promoter to initiate the transcription of both functionally unrelated genes with distinct expression patterns. SSR4 consists of six exons and is approximately 70 kb telomeric to the ALD gene. Although alternative splicing of exon 5 has not been detected in human SSR4, transcript variants missing the region homologous to human exon 5 have been detected in both Xenopus laevis and Mus musculus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SSR4
• Entrez:
  • 6748(Human)
• OMIM:
  • 300090(Human)
• Uniprot:
  • AAH03371(Human)