Siglec-5/Siglec-14 Antibody [Biotin]

Human whole blood monocytes and granulocytes were stained with Human Siglec‑5/14 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF1072, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

• Reactivity: Hu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Siglec-5/Siglec-14 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Siglec-5
  Lys18-Thr434
  Accession # O15389
• Specificity: Detects human Siglec‑5/Siglec‑14 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Siglec‑7 and rhSiglec-9 is observed and less than 5% cross-reactivity with rhSiglec-2 and rhSiglec-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIGLEC5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-5/Siglec-14 Antibody [Biotin]

• CD170;CD33L2;OBBP2;OB-BP2;Sialic acid-binding Ig-like lectin 5;SIGLEC5;SIGLEC-5
• Siglec-5/Siglec-14

Background

Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins (2) belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding (3), followed by varying numbers of Ig-like C2-type domains (1, 4). Eleven human Siglecs have been cloned and characterized (1, 4). They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and the Siglec-5 to 11 (4, 5,  6). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglec-5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (4, 5). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasma tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-5/Siglec-14 Antibody (BAF1072)(3)

Publications using BAF1072

• Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity Sci Rep, 2016-11-28;6(0):37953. 2016-11-28 [PMID: 27892504] (ELISA Development (Detection))
• Zou Z, Chastain A, Moir S Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids. PLoS ONE, 2011-09-08;6(9):e24559. 2011-09-08 [PMID: 21931755] (Flow Cytometry, Human)
• Carlin AF, Chang YC, Areschoug T, Lindahl G, Hurtado-Ziola N, King CC, Varki A, Nizet V Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5. J. Exp. Med., 2009-07-13;206(8):1691-9. 2009-07-13 [PMID: 19596804] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SIGLEC5
• Uniprot:
  • O15389(Human)