Siglec-5/CD170 Overexpression Lysate (Native)

Images

 
Western Blot: SIGLEC5 Overexpression Lysate (Adult Normal) [NBL1-15960] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIGLEC5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Siglec-5/CD170 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206610 and based on accession number NM_003830. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens sialic acid binding Ig-like lectin 5 (SIGLEC5), mRNA.
Gene
SIGLEC5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
60.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Siglec-5/CD170

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Siglec-5/CD170 Overexpression Lysate (Native)

  • CD170 antigen
  • CD170
  • CD33 antigen-like 2
  • CD33L2
  • CD33L2OB binding protein-2
  • OBBP2
  • OB-BP2OB-binding protein 2
  • OBBP2sialic acid-binding Ig-like lectin 5
  • Obesity-binding protein 2
  • sialic acid binding Ig-like lectin 5
  • sialic acid-binding immunoglobulin-like lectin 5
  • Siglec5
  • Siglec-5

Background

The sialic acid-binding immunoglobulin-like lectins (SIGLECs), such as SIGLEC5, are a subgroup of the immunoglobulin (Ig) superfamily that mediate protein-carbohydrate interactions. They specifically interact with sialic acids in glycoproteins and glycolipids, with each SIGLEC having a particular preference for both the nature of the sialic acid and its glycosidic linkage to adjacent sugars. SIGLECs have similar structures, including extracellular Ig-like domains composed of an N-terminal V-set domain followed by varying numbers of C2-set domains. It appears that all SIGLECs have an unusual arrangement of conserved cysteine residues in the V-set and adjacent C2-set domains. Most SIGLECs are expressed uniquely within the hematopoietic system (Cornish et al., 1998 [PubMed 9731071]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Siglec-5/CD170 Lysate (NBL1-15960) (0)

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Bioinformatics

Gene Symbol SIGLEC5
Entrez