HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Siglec‑5/CD170 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human Siglec‑5/CD170 Monoclonal Antibody (Catalog # MAB1072) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1072-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06442-100ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91149-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89986-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99876-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06475-50ul | |||
![Flow Cytometry: Siglec-5/CD170 Antibody (07) [NBP3-06442] - Flow cytometric analysis of Human SIGLEC5(CD170) expression on human whole blood granulocytes. Cells were stained with purified anti-Human SIGLEC5(CD170), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.](http://images.novusbio.com/fullsize/Siglec-5-CD170-Antibody-07-Flow-Cytometry-NBP3-06442-img0001.jpg "Flow Cytometry: Siglec-5/CD170 Antibody (07) [NBP3-06442] - Flow cytometric analysis of Human SIGLEC5(CD170) expression on human whole blood granulocytes. Cells were stained with purified anti-Human SIGLEC5(CD170), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.")
![Immunohistochemistry-Paraffin: Siglec-5/CD170 Antibody [NBP1-91149] - Staining of human stomach shows no cytoplasmic positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Siglec-5-CD170-Antibody-Immunohistochemistry-Paraffin-NBP1-91149-img0008.jpg "Immunohistochemistry-Paraffin: Siglec-5/CD170 Antibody [NBP1-91149] - Staining of human stomach shows no cytoplasmic positivity in glandular cells as expected.")
![Immunohistochemistry-Paraffin: Siglec-5/CD170 Antibody [NBP1-91149] - Staining of human bone marrow shows strong cytoplasm granular positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Siglec-5-CD170-Antibody-Immunohistochemistry-Paraffin-NBP1-91149-img0006.jpg)
![Immunohistochemistry-Paraffin: Siglec-5/CD170 Antibody [NBP1-91149] - Staining of human smooth muscle shows weak cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Siglec-5-CD170-Antibody-Immunohistochemistry-Paraffin-NBP1-91149-img0007.jpg)
![Immunohistochemistry-Paraffin: Siglec-5/CD170 Antibody [NBP1-91149] - Staining of human tonsil shows moderate cytoplasm granular positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Siglec-5-CD170-Antibody-Immunohistochemistry-Paraffin-NBP1-91149-img0009.jpg)
![Flow Cytometry: Siglec-5/CD170 Antibody (1A5) [NBP3-09154] - Surface staining of human peripheral blood cells using anti-CD170 (1A5) purified, GAM-APC.](http://images.novusbio.com/fullsize/Siglec-5-CD170-Antibody-1A5-Flow-Cytometry-NBP3-09154-img0001.jpg "Flow Cytometry: Siglec-5/CD170 Antibody (1A5) [NBP3-09154] - Surface staining of human peripheral blood cells using anti-CD170 (1A5) purified, GAM-APC.")
![Flow Cytometry: Siglec-5/CD170 Antibody (110) [NBP2-89986] - Analysis of Human SIGLEC5(CD170) expression on human whole blood granulocytes. Cells were stained with purified anti-Human SIGLEC5(CD170), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.](http://images.novusbio.com/fullsize/Siglec-5-CD170-Antibody-110-Flow-Cytometry-NBP2-89986-img0001.jpg "Flow Cytometry: Siglec-5/CD170 Antibody (110) [NBP2-89986] - Analysis of Human SIGLEC5(CD170) expression on human whole blood granulocytes. Cells were stained with purified anti-Human SIGLEC5(CD170), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.")
