Human Siglec-5/CD170 ELISA Kit (Colorimetric) Summary
| Sensitivity |
8 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
SIGLEC5 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
SIGLEC5 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SIGLEC5. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample(serum/plasma) diluent.
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culturemedium/urine) diluent.
- Detection Antibody Siglec-5 (Item F): 2 vial of biotinylated anti-human Siglec-5 (each vial is enough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 8 µl 20,000x concentrated HRP-conjugated streptavidin.
- Siglec-5 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human Siglec-5.
- Standards (Item C): 2 vials of recombinant human Siglec-5.
- Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Siglec-5/CD170 ELISA Kit (Colorimetric)
Background
SIGLEC5 - sialic acid binding Ig-like lectin 5
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Siglec-5/CD170 ELISA Kit (KA1765). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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