Human Siglec-5/CD170 ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1765
    • Availability
      Product Discontinued

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Human Siglec-5/CD170 ELISA Kit (Colorimetric) Summary

Sensitivity
8 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SIGLEC5

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
SIGLEC5 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SIGLEC5.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample(serum/plasma) diluent.
  2. Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culturemedium/urine) diluent.
  3. Detection Antibody Siglec-5 (Item F): 2 vial of biotinylated anti-human Siglec-5 (each vial is enough to assay half microplate).
  4. HRP-Streptavidin Concentrate (Item G): 8 µl 20,000x concentrated HRP-conjugated streptavidin.
  5. Siglec-5 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human Siglec-5.
  6. Standards (Item C): 2 vials of recombinant human Siglec-5.
  7. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Siglec-5/CD170 ELISA Kit (Colorimetric)

  • CD170 antigen
  • CD170
  • CD33 antigen-like 2
  • CD33L2
  • CD33L2OB binding protein-2
  • OBBP2
  • OB-BP2OB-binding protein 2
  • OBBP2sialic acid-binding Ig-like lectin 5
  • Obesity-binding protein 2
  • sialic acid binding Ig-like lectin 5
  • sialic acid-binding immunoglobulin-like lectin 5
  • Siglec5
  • Siglec-5

Background

SIGLEC5 - sialic acid binding Ig-like lectin 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Siglec-5/CD170 ELISA Kit (KA1765). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol SIGLEC5