Human Siglec-5/CD170 ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1765
    • Availability
      Product Discontinued

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Human Siglec-5/CD170 ELISA Kit (Colorimetric) Summary

Sensitivity
8 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SIGLEC5

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
SIGLEC5 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SIGLEC5.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample(serum/plasma) diluent.
  2. Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culturemedium/urine) diluent.
  3. Detection Antibody Siglec-5 (Item F): 2 vial of biotinylated anti-human Siglec-5 (each vial is enough to assay half microplate).
  4. HRP-Streptavidin Concentrate (Item G): 8 µl 20,000x concentrated HRP-conjugated streptavidin.
  5. Siglec-5 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human Siglec-5.
  6. Standards (Item C): 2 vials of recombinant human Siglec-5.
  7. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Siglec-5/CD170 ELISA Kit (Colorimetric)

  • CD170 antigen
  • CD170
  • CD33 antigen-like 2
  • CD33L2
  • CD33L2OB binding protein-2
  • OBBP2
  • OB-BP2OB-binding protein 2
  • OBBP2sialic acid-binding Ig-like lectin 5
  • Obesity-binding protein 2
  • sialic acid binding Ig-like lectin 5
  • sialic acid-binding immunoglobulin-like lectin 5
  • Siglec5
  • Siglec-5

Background

SIGLEC5 - sialic acid binding Ig-like lectin 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Siglec-5/CD170 ELISA Kit (KA1765). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol SIGLEC5