Siglec-5/CD170 Antibody (194117) [Unconjugated]

Siglec‑5/CD170 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human Siglec‑5/CD170 Monoclonal Antibody (Catalog # MAB1072) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 194117
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Siglec-5/CD170 Antibody (194117) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Siglec‑5/CD170
  Lys18-Thr434
  Accession # O15389
• Specificity: Detects human Siglec‑5/CD170 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Siglec-2, -3, -7, -9, or -14 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIGLEC5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-5/CD170 Antibody (194117) [Unconjugated]

• CD170 antigen
• CD170
• CD33 antigen-like 2
• CD33L2
• CD33L2OB binding protein-2
• OBBP2
• OB-BP2OB-binding protein 2
• OBBP2sialic acid-binding Ig-like lectin 5
• Obesity-binding protein 2
• sialic acid binding Ig-like lectin 5
• sialic acid-binding immunoglobulin-like lectin 5
• Siglec5
• Siglec-5

Background

Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins (2) belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding (3), followed by varying numbers of Ig-like C2-type domains (1, 4). Eleven human Siglecs have been cloned and characterized (1, 4). They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and the Siglec-5 to 11 (4, 5, 7). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglec-5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (4, 5). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasma tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Human Siglec-5 cDNA encodes a 551 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, three Ig-like C2-type domains, a transmembrane region and a cytoplasma tail (6). Siglec-5 exists as a disulfide-linked homodimer on the cell surface and is expressed on monocytes, neutrophils and B cells (4, 5, 6). It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid (6).

1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
2. Powell, L.D. et al. (1995) J. Biol. Chem. 270:14243.
3. May, A.R. et al. (1998) Mol. Cell 1998. 1:719.
4. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
5. Crocker, P.R. et al. (2001) Immunology 103:137.
6. Cornish, A.L. et al. (1998) Blood 92:2123.
7. Angata, T. et al. (2002) J. Biol Chem. 277:24466.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-5/CD170 Antibody (MAB1072)(5)

Publications using MAB1072

• N Rochereau, E Michaud, L Waeckel, M Killian, R Gayet, R Goguyer-De, X Roblin, G Biolley, B Corthésy, S Paul Essential role of TOSO/FAIM3 in intestinal IgM reverse transcytosis Cell Reports, 2021-11-16;37(7):110006. 2021-11-16 [PMID: 34788614] (Neutralization, Human)
• K Alkhodair, H Almhanna, J McGetrick, S Gedair, ME Gallagher, B Fernandez-, T Tharmaling, PB Larsen, E Fitzpatric, P Lonergan, ACO Evans, SD Carrington, CJ Reid Siglec expression on the surface of human, bull and ram sperm Reproduction, 2018-04-01;155(4):361-371. 2018-04-01 [PMID: 29581386] (IHC, Western Blot, Bovine, Human, Ovine)
• Ali S, Fong J, Carlin A, Busch T, Linden R, Angata T, Areschoug T, Parast M, Varki N, Murray J, Nizet V, Varki A Siglec-5 and Siglec-14 are polymorphic paired receptors that modulate neutrophil and amnion signaling responses to group B Streptococcus. J Exp Med, 2014-05-05;211(6):1231-42. 2014-05-05 [PMID: 24799499] (Western Blot, Human)
• Nordstrom T, Movert E, Olin AI, Ali SR, Nizet V, Varki A, Areschoug T Human Siglec-5 inhibitory receptor and immunoglobulin A (IgA) have separate binding sites in streptococcal beta protein. J. Biol. Chem., 2011-07-27;286(39):33981-91. 2011-07-27 [PMID: 21795693] (Western Blot, Human)
• Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. FASEB J., 2006-10-01;20(12):1964-73. 2006-10-01 [PMID: 17012248] (ICC, Human)

Bioinformatics

• Gene Symbol: SIGLEC5
• Uniprot:
  • O15389(Human)