Siglec-2/CD22 Antibody (4KB128) [FITC]

Product Discontinued

Siglec-2/CD22 Antibody (4KB128) [FITC] Summary

• Immunogen: The immunogen for this antibody was CD22.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD22
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: This 4KB128 (4KB128) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 ul (0.25 ug)/per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS [pH 7.2] containing BSA and/or gelatin for protein stabilization
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Siglec-2/CD22 Antibody (4KB128) [FITC]

• B-cell receptor CD22
• BL-CAM
• B-lymphocyte cell adhesion molecule
• CD22 antigenMGC130020
• CD22 molecule
• CD22
• sialic acid binding Ig-like lectin 2
• Sialic acid-binding Ig-like lectin 2
• Siglec2
• Siglec-2
• SIGLEC2FLJ22814
• T-cell surface antigen Leu-14

Background

The 4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), which is a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains, CD22alpha and CD22beta, of 130 and 140 kDa respectively, produced by alternative splicing of the CD22 gene. CD22 is expressed at high levels on mature B cells and B cell lymphomas. The extracellular portion of CD22 contains seven Ig-like domains, some of which are capable of binding ligands with sialic acid moieties expressed on epithelial, endothelial, B and T cells. The intracellular portion of CD22 contains 6 tyrosine residues contained within immunotyrosine-based inhibitory motifs (ITIM) and immunotyrosine-based activation-like motifs, which are phosphorylated upon B-cell receptor engagement, which enables CD22 to participate in the positive and negative regulation of B-cell receptor signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Siglec-2/CD22 Antibody (NBP1-44025). (Showing 1 - 1 of 1 FAQ).

1. I am interested in buying a CD22 antibody for fluorescence microscopy and FACS I red on your web page that you also offer to label your antibodies, if required, is that correct?
   • We do have the ability to perform custom conjugations on certain antibodies, including some to the CD22 protein. If you send me the conjugate you are interested in I can provide you with a quote.

Bioinformatics

• Gene Symbol: CD22