Siglec-1/CD169 Antibody (ED3) [Alexa Fluor® 532]

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr
Clone
ED3
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 532

Order Details

Siglec-1/CD169 Antibody (ED3) [Alexa Fluor® 532] Summary

Immunogen
Rat Spleen cell homogenate
Specificity
NB100-65301 recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Siglec-1/CD169 Antibody (ED3) [Alexa Fluor® 532]

  • CD169
  • FLJ00051
  • sialic acid binding Ig-like lectin 1, sialoadhesin
  • sialoadhesin
  • Siglec1
  • Siglec-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Siglec-1/CD169 Antibody (NB100-65301AF532) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-65301AF350
Alexa Fluor 405 NB100-65301AF405
Alexa Fluor 488 NB100-65301AF488
Alexa Fluor 532 NB100-65301AF532
Alexa Fluor 594 NB100-65301AF594
Alexa Fluor 647 NB100-65301AF647
Alexa Fluor 700 NB100-65301AF700
Alexa Fluor 750 NB100-65301AF750
Allophycocyanin NB100-65301APC
Allophycocyanin/Cy7 NB100-65301APCCY7
DyLight 350 NB100-65301UV
DyLight 405 NB100-65301V
DyLight 488 NB100-65301G
DyLight 550 NB100-65301R
DyLight 594 NB100-65301DL594
DyLight 650 NB100-65301C
DyLight 680 NB100-65301FR
DyLight 755 NB100-65301IR
Janelia Fluor 549 NB100-65301JF549
Janelia Fluor 646 NB100-65301JF646
PE NB100-65301PE
PE/Atto594 NB100-65301PEATT594
PE/Cy5.5 NB100-65301PECY55
PE/Cy7 NB100-65301PECY7
PerCP NB100-65301PCP

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Bioinformatics Tool for Siglec-1/CD169 Antibody (NB100-65301AF532)

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Bioinformatics

Gene Symbol SIGLEC1