SIAH2 Antibody

Product Discontinued

SIAH2 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 241-290 of Human Siah-2.
• Specificity: Siah-2 (E266) detects endogenous levels of Siah-2 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIAH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Siah-2 (E266) pAb in extracts from COLO cells.
  Immunohistochemistry (IHC) analyzes of Siah-2 (E266) pAb in paraffin-embedded human heart tissue.
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SIAH2 Antibody

• E3 ubiquitin-protein ligase SIAH2
• EC 6.3.2
• EC 6.3.2.-
• hSiah2
• seven in absentia (Drosophila) homolog 2
• seven in absentia homolog 2 (Drosophila)
• Seven in absentia homolog 2
• siah-2

Background

SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkappaB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIAH2 Antibody (NBP1-19648)(3)

Publications using NBP1-19648

• Yan T, Zhou D, Shi Y et al. Targeting ADT-Induced Activation of the E3 Ubiquitin Ligase Siah2 to Delay the Occurrence of Castration-Resistant Prostate Cancer Frontiers in Oncology 2021-04-16 [PMID: 33937036] (WB, Human)
• Liao Y, Zhang M, Lonnerdal B. Growth factor TGF-? induces intestinal epithelial cell (IEC-6) differentiation: miR-146b as a regulatory component in the negative feedback loop. Genes Nutr. 2012-05-09 [PMID: 22570175] (WB, Rat)
• Kilroy G, Kirk-Ballard H, Carter LE, Floyd ZE. The Ubiquitin Ligase Siah2 Regulates PPAR? Activity in Adipocytes. Endocrinology. 2012-01-31 [PMID: 22294748] (WB, Mouse)

Bioinformatics

• Gene Symbol: SIAH2
• Entrez:
  • 6478(Human)
  • 20439(Mouse)
  • 140593(Rat)
• Uniprot:
  • O43255(Human)