SHP-2/PTPN11 Antibody (255509) [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu, Rt
• Clone: 255509
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 647

SHP-2/PTPN11 Antibody (255509) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human, mouse, and rat SHP-2 in Western blots. In Western blots, no cross-reactivity with recombinant human SHP-1 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SHP-2/PTPN11 Antibody (255509) [Alexa Fluor™ Plus 647]

• BPTP3
• EC 3.1.3.48
• MGC14433
• protein tyrosine phosphatase, non-receptor type 11
• protein tyrosine phosphatase-2
• Protein-tyrosine phosphatase 1D
• Protein-tyrosine phosphatase 2C
• PTP1D
• PTP-1D
• PTP2C
• PTP-2C
• PTP2CNS1
• PTPN11
• SHP2
• SHP-2
• SHP2CFC
• SHPTP2
• SH-PTP2
• SH-PTP2Noonan syndrome 1
• SH-PTP3
• tyrosine-protein phosphatase non-receptor type 11

Background

Src-Homology domain-2 containing protein tyrosine Phosphatase 2 (SHP-2), also called protein tyrosine phosphatase, non-receptor type 11 (PTPN11), PTP1D, PTP2C, and SYP, is an enzyme that dephosphorylates tyrosine residues in proteins. The protein contains two Src homology 2 (SH2) domains, which both regulate the activity of the enzyme (1) and allow it to selectively bind to SH2 sites on proteins such as Dok1, IRS1, and the insulin receptor (2). SHP-2 plays a unique stimulatory role in cell signaling. Cells lacking SHP-2 have poor mobility because the hyper-phosphorylation of FAK and other proteins in the focal adhesion complex (3) prevents turnover of cellular attachment points. Without SHP-2, sustained ERK stimulation does not take place (4). The Y992 phosphorylation site of EGFR is a particularly good substrate for SHP-2 (5).

1. Zhao, Z. et al. (1994) J. Biol. Chem. 269:8780.
2. Clemmons, D.R. and Maile, L.A. (2005) Mol. Endocrinol. 19:1.
3. von Wichert, G. et al. (2003) EMBO J. 22:5023.
4. Maroun, C.R. et al. (2000) Mol. Cell. Biol. 20:8513.
5. Sugimoto, S. et al. (1993) J. Biol. Chem. 269:22771.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.