SHIP Antibody [Unconjugated]

Western blot shows lysates of BaF3 mouse pro-B cell line and RL human non-Hodgkin's lymphoma B cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SHIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SHIP at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PMA-treated U-937 lysates were prepared and immunoprecipitation was performed using 2.0 µg of Goat Anti-Human/Mouse SHIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10117) pre-coupled to Dynabeads protein G. Immunoprecipitated SHIP was detected with Mouse Anti-SHIP Antibody.The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

SHIP Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse SHIP
  Glu874-Thr941
  Accession # Q9ES52
• Specificity: Detects mouse SHIP in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 ug/1 mg cell lysate
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SHIP Antibody [Unconjugated]

• 145kD
• EC 3.1.3
• hp51CNMGC142142
• Inositol polyphosphate-5-phosphatase of 145 kDa
• inositol polyphosphate-5-phosphatase, 145kDa
• INPP5D
• MGC104855
• SHIP
• SHIPMGC142140

Background

SH2-containing inositol phosphatase (SHIP), also known as INPP5D, is a negative regulator of signal transduction in hematopoietic cells. Targeted disruption of SHIP in mice leads to a myeloproliferative disorder. Several laboratories have demonstrated the presence of multiple forms of SHIP, including 145 kDa, 135 kDa, and C-terminal truncated forms at 125 kDa and 110 kDa in some cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9ES52()