SHIP Antibody (SHIP-02) - BSA Free

Flow Cytometry: Mouse Monoclonal SHIP Antibody (SHIP-02) [NBP1-45077] - Separation of MOLT-4 cells stained using anti-SHIP1 (SHIP-02) purified antibody (concentration in sample 3 μg/ml, GAM APC, red-filled) from MOLT-4 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Western Blot: SHIP Antibody (SHIP-02) [NBP1-45077] - Analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line. Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-04) Lane 2,3: immunostaining with anti-human SHIP-1 (SHIP-01; cat. no. NB110-81538) Lane 4,5: immunostaining with anti-human SHIP-1 (SHIP-02)

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: SHIP-02
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SHIP Antibody (SHIP-02) - BSA Free Summary

• Immunogen: Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
• Specificity: The antibody SHIP-02 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: INPP5D
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular) 2-5 ug/ml
  • Western Blot 1:1000
• Application Notes: Western Blotting - Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 6 min. Application note:. This antibody is CyTOF ready.
• Theoretical MW: 145 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SHIP Antibody (SHIP-02) - BSA Free

• 145kD
• EC 3.1.3
• hp51CNMGC142142
• Inositol polyphosphate-5-phosphatase of 145 kDa
• inositol polyphosphate-5-phosphatase, 145kDa
• INPP5D
• MGC104855
• SHIP
• SHIPMGC142140

Background

SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5'inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INPP5D