SHIP Antibody (SHIP-02)

Images

 
Western Blot: SHIP Antibody (SHIP-02) [NBP1-45077] - Analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line. Lane 1: immunostaining with Isotype mouse IgG1 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
SHIP-02
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

SHIP Antibody (SHIP-02) Summary

Immunogen
Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
Specificity
The antibody SHIP-02 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
INPP5D
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 2 - 5 ug/ml
  • CyTOF-ready
Application Notes
Western Blotting - Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% llaurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 6 min. Application note:

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for SHIP Antibody (SHIP-02)

  • 145kD
  • EC 3.1.3
  • hp51CNMGC142142
  • Inositol polyphosphate-5-phosphatase of 145 kDa
  • inositol polyphosphate-5-phosphatase, 145kDa
  • INPP5D
  • MGC104855
  • SHIP
  • SHIPMGC142140

Background

SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5'inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SHIP Antibody (NBP1-45077) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-45077AF405
Alexa Fluor 488 Labeled NBP1-45077AF488
Alexa Fluor 647 Labeled NBP1-45077AF647
Alexa Fluor 700 Labeled NBP1-45077AF700
Allophycocyanin Labeled NBP1-45077APC
DyLight 350 Labeled NBP1-45077UV
DyLight 405 Labeled NBP1-45077V
DyLight 488 Labeled NBP1-45077G
DyLight 680 Labeled NBP1-45077FR
DyLight 755 Labeled NBP1-45077IR
PE Labeled NBP1-45077PE
PerCP Labeled NBP1-45077PCP

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Bioinformatics Tool for SHIP Antibody (NBP1-45077)

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Blogs on SHIP.

Getting SHIP-shape Over Tumour Suppression
PTEN antibodies have shown PTEN to be an important tumor suppressor and, in mutated form, a factor in cancer development. However, a recent study, led by Robert Rickert, shows that the SHIP gene may also be an important tumor suppressor in B-cell lymp...  Read full blog post.

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Bioinformatics

Gene Symbol INPP5D
Entrez