SHANK1 Antibody (S22-21)

Images

 
Western Blot: SHANK1 Antibody (S22-21) [NBP2-12915] - analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21. Load: 15 ug protein. Block: 1.5% ...read more
Immunohistochemistry-Paraffin: SHANK1 Antibody (S22-21) [NBP2-12915] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody at 1:100 ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
S22-21
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SHANK1 Antibody (S22-21) Summary

Immunogen
Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1.
Localization
Cytoplasm, Cell Junction, Synapse, Postsynaptic Cell Membrane, Postsynaptic Density
Specificity
Detects approx 190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SHANK1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
Application Notes
1 ug/ml of SHANK1 Antibody was sufficient for detection of Shank1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Based on homology, It's predicted to detect Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SHANK1 Antibody (S22-21)

  • SH3 and multiple ankyrin repeat domains 1
  • SH3 and multiple ankyrin repeat domains protein 1
  • Shank1
  • Somatostatin receptor-interacting protein
  • SSTR-interacting protein
  • SSTRIPSPANK-1
  • synamon

Background

1 ug/mL was sufficient for detection of Shank1 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SHANK1