SHANK1 Antibody (N22/21) [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, MA
• Clone: N22/21
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

SHANK1 Antibody (N22/21) [Alexa Fluor™ Plus 647] Summary

• Immunogen: Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1
• Localization: Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density
• Specificity: Detects approx 190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SHANK1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Microarray
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SHANK1 Antibody (N22/21) [Alexa Fluor™ Plus 647]

• SH3 and multiple ankyrin repeat domains 1
• SH3 and multiple ankyrin repeat domains protein 1
• Shank1
• Somatostatin receptor-interacting protein
• SSTR-interacting protein
• SSTRIPSPANK-1
• synamon

Background

Shank is a novel family of postsynaptic density proteins (PSD) that bind to PSD-95-associated protein GKAP. Its putative function is to act as a scaffold protein in the PSD to cross-link NMDA receptors/PSD-95 complexes and couple them to regulators of the actin cytoskeleton. Shank1a is a splice variant of Shank1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHANK1