SH2B1 Antibody [Alexa Fluor® 594]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

SH2B1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human SH2B1
  Pro317-Leu467
  Accession # Q9NRF2
• Specificity: Detects human, mouse, and rat SH2B1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SH2B1 Antibody [Alexa Fluor® 594]

• FLJ30542
• KIAA1299
• Pro-rich, PH and SH2 domain-containing signaling mediator
• PSM
• SH2 domain-containing protein 1B
• SH2 domain-containing putative adapter SH2-B
• SH2B adapter protein 1
• SH2B adaptor protein 1
• SH2-B signaling protein
• SH2B1
• SH2BDKFZp547G1110

Background

SH2B1 (Src-Homology 2 Domain-Containing protein B1/1B; also SH2B1 alpha , PSM and SH2B adaptor protein 1) is a member of the SH2B adapter family of proteins. Although its predicted MW is 80 kDa, it runs anomalously at 90-102 kDa in SDS-Page. SH2B1 is a nucleocytoplasmic protein that is widely expressed in cells such as skeletal muscle cells, neurons, and adipocytes. It serves as a linker between Jak2 and multiple downstream molecules such as Rac and IRS1. It does this following phosphorylation on a number of potential sites. It also promotes the enzymatic activity of associated receptor kinases, thus potentiating ligand-receptor interactions. SH2B1 appears to bind to nonphosphorylated Jaks as a monomer, while Jak phosphorylation induces SH2B1 dimerization. SH2B1 is further reported to form a homopentamer, and to oligomerize with SH2B2. Human SH2B1( alpha ) is 756 amino acids amino acids (aa) in length. It contains one dimerization segment (aa 24-85), an NLS (aa 224-233), one pleckstrin homology region (aa 249-378) and an SH2 domain (aa 521-625). At least three utilized phosphorylation sites exist. There are at least two splice variants for SH2B1. The 90-92 kDa SH2B1 beta isoform shows a 39 aa substitution for aa 633-756, while the SH2B1 gamma isoform possesses a 50 aa substitution for the same aa range (i.e., aa 633-756). Over aa 317-467, human SH2B1 shares 92% aa identity with mouse SH2B1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9NRF2()