sFRP-4 Antibody (JE41-41)

Western Blot: sFRP-4 Antibody (JE41-41) [NBP2-76870] - Western blot analysis of SFRP4 on different lysates using anti-SFRP4 antibody at 1/1,000 dilution.Positive control:Lane 1: Mouse testis Lane 2: A549Lane 3: HepG2 Lane 4: SH-SY-5Y

Flow Cytometry: sFRP-4 Antibody (JE41-41) [NBP2-76870] - Flow cytometric analysis of A549 cells with SFRP4 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product Discontinued

sFRP-4 Antibody (JE41-41) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human sFRP-4 aa 181-320 / 346. (SwissProt: Q6FHJ7 Human; SwissProt: Q9Z1N6 Mouse; SwissProt: Q9JLS4 Rat)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SFRP4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for sFRP-4 Antibody (JE41-41)

• DDC-4
• Frizzled protein, human endometrium
• FRP-4
• FRP-AP
• frpHE
• FRPHEMGC26498
• FrzB2
• secreted frizzled-related protein 4
• secreted frizzled-related protein 4; secreted frizzled-related protein 4
• sFRP4
• sFRP-4

Background

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SFRP4