sFRP-2 Antibody (755644)

Western blot shows lysates of human adipose tissue and human lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human sFRP-2 Monoclonal Antibody (Catalog # MAB6838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for sFRP-2 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 755644
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

sFRP-2 Antibody (755644) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human sFRP-2
  Leu25-Cys295
  Accession # Q96HF1
• Specificity: Detects human sFRP-2 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) sFRP-2 is observed and no cross-reactivity with recombinant human (rh) sFRP-1 or rhsFRP-5 is observed. In Western blots, no cross-reactivity with rhsFRP-1, -4, -5, or rmsFRP-2 is observed. 100% cross-reactivity with rhsFRP-3 is observed under non-reducing conditions only.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SFRP2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for sFRP-2 Antibody (755644)

• FRP2
• FRP-2secreted apoptosis related protein 1
• SARP-1
• SARP1sFRP-2
• SDF-5
• Secreted apoptosis-related protein 1
• secreted frizzled-related protein 2
• sFRP2
• sFRP-2

Background

Secreted Frizzled Related Protein-2 (sFRP-2) belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30‑35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C‑terminal domain (1, 2). Mature human sFRP-2, also known as SARP-1, SDF-5, and FRP-2, shares 99% aa sequence identity with mouse and rat sFRP-2 (3). sFRP‑2 is widely expressed during embryogenesis and in the adult in tissues including the eye, heart, lung, colon, intestine, smooth muscle, pancreas, prostate, testis, kidney, brain, teeth and joints, craniofacial mesenchyme, and preadipocytes (3‑6). Depending on the context, sFRP-2 can exert either positive or negative effects on Wnt signaling (7‑9). It also inhibits BMP-induced effects (8, 10). sFRP-2 can be incorporated into the extracellular matrix through interactions with Fibronectin and Integrin alpha 5 beta 1 (11). sFRP-2 plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation (8, 10, 12, 13). sFRP-2 is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity (10, 14). At physiological concentrations sFRP-2 enhances BMP-1 mediated proteolysis of Pro-Collagen I, whereas at higher concentrations it inhibits BMP-1 activity (15, 16). This difference is significant considering that sFRP-2 is up‑regulated in fibrotic areas of the heart following myocardial infarction (15, 16). Elevated levels of sFRP-2 then promote the recovery of cardiac function by reducing collagen deposition, remodeling, and calcification and by promoting the engraftment of mesenchymal stem cells into the heart (8, 15).

1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
3. Hu, E. et al. (1998) Biochem. Biophys. Res. Commun. 247:287.
4. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
5. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
6. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
7. Galli, L.M. et al. (2006) Dev. Dyn. 235:681.
8. Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645.
9. von Marschall, Z. and L.W. Fisher (2010) Biochem. Biophys. Res. Commun. 400:299.
10. Oshima, T. et al. (2005) Blood 106:3160.
11. Lee, J.-L. et al. (2004) J. Biol. Chem. 279:14602.
12. Sugiyama, Y. et al. (2010) Dev. Biol. 338:193.
13. Descamps, S. et al. (2008) Cell Tissue Res. 332:299.
14. Roth, W. et al. (2000) Oncogene 19:4210.
15. He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110.
16. Kobayashi, K. et al. (2009) Nat. Cell Biol. 11:46.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for sFRP-2 Antibody (MAB6838)(3)

Publications using MAB6838

• Lewis, PA;Silajdži?, E;Smith, H;Bates, N;Smith, CA;Mancini, FE;Knight, D;Denning, C;Brison, DR;Kimber, SJ; A secreted proteomic footprint for stem cell pluripotency PloS one 2024-06-14 [PMID: 38875182] (Western Blot, Human)
• Fane, M E, Ecker, B L Et al. sFRP2 Supersedes VEGF as an Age-related Driver of Angiogenesis in Melanoma, Affecting Response to Anti-VEGF Therapy in Older Patients. Clin Cancer Res 11/1/2020 [PMID: 33097493]
• A Kaur, MR Webster, K Marchbank, R Behera, A Ndoye, CH Kugel, VM Dang, J Appleton, MP O'Connell, P Cheng, AA Valiga, R Morissette, NB McDonnell, L Ferrucci, AV Kossenkov, K Meeth, HY Tang, X Yin, WH Wood, E Lehrmann, KG Becker, KT Flaherty, DT Frederick sFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance Nature, 2016-04-04;532(7598):250-4. 2016-04-04 [PMID: 27042933] (ELISA Development (Detection), Human)

Bioinformatics

• Gene Symbol: SFRP2
• Uniprot:
  • Q96HF1()