SF3A3 Overexpression Lysate (Native)

Images

 
Western Blot: SF3A3 Overexpression Lysate (Adult Normal) [NBL1-15875] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SF3A3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SF3A3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203895 and based on accession number NM_006802. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens splicing factor 3a, subunit 3, 60kDa (SF3A3), mRNA.
Gene
SF3A3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
58.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SF3A3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SF3A3 Overexpression Lysate (Native)

  • PRP9
  • PRPF9
  • SAP 61
  • SAP61splicing factor 3a, subunit 3, 60kD
  • SF3a60pre-mRNA splicing factor SF3a (60kD)
  • spliceosome associated protein 61
  • Spliceosome-associated protein 61
  • splicing factor 3A subunit 3
  • splicing factor 3a, subunit 3, 60kDa

Background

This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SF3A3 Lysate (NBL1-15875) (0)

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Bioinformatics

Gene Symbol SF3A3