SF3A2 Antibody (3B6) - Azide and BSA Free

Western Blot: SF3A2 Antibody (3B6) [H00008175-M01] - SF3A2 expression in NIH/3T3 ( Cat # L018V1 ).

Immunocytochemistry/Immunofluorescence: SF3A2 Antibody (3B6) [H00008175-M01] - Analysis of monoclonal antibody to SF3A2 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: SF3A2 Antibody (3B6) [H00008175-M01] - SF3A2 expression in PC-12 ( Cat # L012V1 ).

Western Blot: SF3A2 Antibody (3B6) [H00008175-M01] - SF3A2 expression in Hela NE ( Cat # L013V3 ).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP, MA
• Clone: 3B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SF3A2 Antibody (3B6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SF3A2 (NP_009096, 112 a.a. ~ 216 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IGRPGYKVTKQRDSEMGQQSLLFQIDYPEIAEGIMPRHRFMSAYEQRIEPPDRRWQYLLMAAEPYETIAFKVPSREIDKAEGKFWTHWNRETKQFFLQFHFKMEK
• Specificity: SF3A2 (3B6)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SF3A2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Protein Array
  • Western Blot
• Application Notes: This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA. IP usage reported in scientific literature (PMID: 22672905).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SF3A2 Antibody (3B6) - Azide and BSA Free

• pre-mRNA splicing factor SF3A, subunit 2
• PRP11
• PRPF11
• SAP 62
• SAP62Prp11
• SF3a66splicing factor 3a, subunit 2, 66kD
• spliceosome associated protein 62
• Spliceosome-associated protein 62
• splicing factor 3A subunit 2
• splicing factor 3a, subunit 2, 66kDa

Background

This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SF3A2 Antibody (H00008175-M01)(1)

Publication using H00008175-M01

• Huang Y, Jeong JS, Okamura J et al. Global tumor protein p53/p63 interactome: making a case for cisplatin chemoresistance. Cell Cycle 2012-06-15 [PMID: 22672905] (IP, WB, Human)

Bioinformatics

• Gene Symbol: SF3A2
• Entrez:
  • 8175(Human)
• OMIM:
  • 600796(Human)
• Uniprot:
  • NP_009096(Human)