SEZ6L2/BSRP-A Antibody

SEZ6L2/BSRP-A was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse SEZ6L2/BSRP-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4916) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain (medulla oblongata), spinal cord, trigeminal ganglia, and retina. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SEZ6L2/BSRP-A Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L2/BSRP-A
  Leu28-Ser837
  Accession # Q4V9Z5
• Specificity: Detects human and mouse SEZ6L2/BSRP-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse (rm) BSRP-B and rmBSRP-C is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SEZ6L2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SEZ6L2/BSRP-A Antibody

• BSRPA
• BSRP-A
• PSK-1
• seizure 6-like protein 2
• seizure related 6 homolog (mouse)-like 2
• SEZ6L2
• UNQ1903/PRO4349

Background

SEZ6L2 (Brain-specific receptor-like protein A; also BSRP-A) is a 170 kDa member of the BSRP family of proteins. It is expressed in the plasma membrane and ER of CNS neurons and likely regulates PKC alpha activation. Mature mouse SEZ6L2 is an 890 amino acid (aa) type I transmembrane protein. It contains an 824 aa extracellular domain (ECD) (aa 21-844) and a 43 aa cytoplasmic tail. The ECD shows two CUB domains and five short consensus repeats (SCRs). There are multiple isoforms for SEZ6L2. One shows a premature truncation after Arg403, a second shows a deletion of aa 71-130, and a third likely represents the full-length form that contains a 13 aa insertion after Val830. Over aa 28-837, mouse SEZ6L2 is 96% and 99% aa identical to human and rat SEZ6L2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SEZ6L2/BSRP-A Antibody (AF4916)(7)

Publications using AF4916

• A Nash, HJM Gijsen, BJ Hrupka, KS Teng, SF Lichtentha, H Takeshima, JM Gunnersen, KM Munro BACE inhibitor treatment of mice induces hyperactivity in a Seizure-related gene 6 family dependent manner without altering learning and memory Scientific Reports, 2021-07-23;11(1):15084. 2021-07-23 [PMID: 34302009] (Western Blot, Human)
• Wen Q. Qiu, Shaopeiwen Luo, Stefanie A. Ma, Priyanka Saminathan, Herman Li, Jenny M. Gunnersen et al. The Sez6 Family Inhibits Complement by Facilitating Factor I Cleavage of C3b and Accelerating the Decay of C3 Convertases Frontiers in Immunology 4/15/2021 [PMID: 33936031]
• Martina Pigoni, Hung‐En Hsia, Jana Hartmann, Jasenka Rudan Njavro, Merav D Shmueli, Stephan A Müller et al. Seizure protein 6 controls glycosylation and trafficking of kainate receptor subunits GluK2 and GluK3 The EMBO Journal 8/3/2020 [PMID: 32567721]
• A Shahapal, EB Cho, HJ Yong, I Jeong, H Kwak, JK Lee, W Kim, B Kim, HC Park, WS Lee, H Kim, JI Hwang, JY Seong FAM19A5 Expression During Embryogenesis and in the Adult Traumatic Brain of FAM19A5-LacZ Knock-in Mice Front Neurosci, 2019-08-30;13(0):917. 2019-08-30 [PMID: 31543758] (IHC, Mouse)
• Martina Pigoni, Johanna Wanngren, Peer-Hendrik Kuhn, Kathryn M. Munro, Jenny M. Gunnersen, Hiroshi Takeshima et al. Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neurons Molecular Neurodegeneration 12/1/2016 [PMID: 27716410]
• Marielle Boonen, Catherine Staudt, Florentine Gilis, Viola Oorschot, Judith Klumperman, Michel Jadot Cathepsin D and its newly identified transport receptor Sez6l2 can modulate neurite outgrowth Journal of Cell Science 2/1/2016 [PMID: 26698217]
• Marielle Boonen, Catherine Staudt, Florentine Gilis, Viola Oorschot, Judith Klumperman, Michel Jadot Cathepsin D and its newly identified transport receptor Sez6l2 can modulate neurite outgrowth Journal of Cell Science 2016-02-01 [PMID: 26698217] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: SEZ6L2
• Uniprot:
  • Q4V9Z5(Mouse)