SET Antibody

Western Blot: SET Antibody [NB100-817] - Staining (0.01 ug/ml) of Daudi (A) and Molt4 (B) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: SET Antibody [NB100-817] - Staining (03ug/ml) of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: SET Antibody [NB100-817] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Immunohistochemistry-Paraffin: SET Antibody [NB100-817] - (7ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SET Antibody Summary

• Description: Novus Biologicals Goat SET Antibody (NB100-817) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-SET Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence SAPAAKVSKKELNS-C corresponding to N-Terminus according to NP_003002.2.
• Specificity: This antibody is expected to recognize isoform 2 (NP_003002.2).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SET
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 7 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 38 kDa band observed in lysates of human kidney lysates and of cell lines Daudi and Molt4 (calculated MW of 32.1 kDa band according to NP_003002.2).
• Control:

  SET Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SET Antibody

• 2PP2A
• HLA-DR-associated protein II
• I2PP2A
• I-2PP2A
• IGAAD
• Inhibitor of granzyme A-activated DNase
• inhibitor-2 of protein phosphatase-2A
• IPP2A2
• PHAPIITAF-I
• Phosphatase 2A inhibitor I2PP2A
• protein phosphatase type 2A inhibitor
• protein SET
• SET nuclear oncogene
• SET translocation (myeloid leukemia-associated)
• TAF-IBETA
• Template-activating factor I
• Template-Activating Factor-I, chromatin remodelling factor

Background

SET belongs to a family of multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two named isoforms produced by alternative splicing : Isoform 1 and Isoform 2. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SET Antibody (NB100-817)(2)

Publications using NB100-817

• Li H, Liu L, Chang H et al. Downregulation of MCL-1 and upregulation of PUMA using mTOR inhibitors enhance antitumor efficacy of BH3 mimetics in triple-negative breast cancer Cell Death Dis 2018-01-26 [PMID: 29374168]
• Ozbun LL, You L, Kiang S et al. Identification of differentially expressed nucleolar TGF-beta1 target (DENTT) in human lung cancer cells that is a new member of the TSPY/SET/NAP-1 superfamily. Genomics 2001-04-15 [PMID: 11318608]

Bioinformatics

• Gene Symbol: SET
• Entrez:
  • 6418(Human)
• Uniprot:
  • AAC50460(Human)