SET Overexpression Lysate

Images

 
Western Blot: SET Overexpression Lysate (Adult Normal) [NBL1-15867] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SET.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SET Overexpression Lysate Summary

Description
Transient overexpression lysate of SET nuclear oncogene (SET), transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC207606 and based on accession number NM_003011. The protein contains a C-MYC/DDK Tag.
Gene
SET

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
31.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SET Overexpression Lysate

  • 2PP2A
  • HLA-DR-associated protein II
  • I2PP2A
  • I-2PP2A
  • IGAAD
  • Inhibitor of granzyme A-activated DNase
  • inhibitor-2 of protein phosphatase-2A
  • IPP2A2
  • PHAPIITAF-I
  • Phosphatase 2A inhibitor I2PP2A
  • protein phosphatase type 2A inhibitor
  • protein SET
  • SET nuclear oncogene
  • SET translocation (myeloid leukemia-associated)
  • TAF-IBETA
  • Template-activating factor I
  • Template-Activating Factor-I, chromatin remodelling factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SET Lysate (NBL1-15867) (0)

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Video Protocols

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Other Available Formats

NBL1-15867

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Bioinformatics

Gene Symbol SET
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