Serum Amyloid A4 Antibody (398104)

Serum Amyloid A4 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Mouse Serum Amyloid A4 Monoclonal Antibody (Catalog # MAB4276) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clone: 398104
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Serum Amyloid A4 Antibody (398104) Summary

• Immunogen: E.coli-derived recombinant mouse Serum Amyloid A4
  Asp19-Phe130
  Accession # P31532
• Specificity: Detects mouse Serum Amyloid A4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Serum Amyloid A1, rhSerum Amyloid A4, or recombinant mouse Serum Amyloid A1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SAA4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serum Amyloid A4 Antibody (398104)

• C-SAA
• C-SAACSAAConstitutively expressed serum amyloid A protein
• SAA4
• SAA5
• serum amyloid A-4 protein
• Serum Amyloid A4
• serum amyloid A4, constitutive

Background

Mouse Serum Amyloid A4 (SAA4; also SAA5) is a 14 kDa member of the SAA family of proteins.  It is a constitutively produced apolipoprotein component of HDL that may mediate HDL-VLDL interactions.  Mature mouse SAA4 is 112 amino acids (aa) in length.  Although human SAA4 is glycosylated, mouse SAA4 is not.  Human SAA4 also shows truncation of its Lys-Lys-Tyr C-terminus. The mouse C-terminus is distantly related with a Glu-Lys-Phe tripeptide, but it is unknown if it is proteolytically processed. Over aa 19-130, mouse SAA4 shares 57% and 85% aa identity with mature human and rat SAA4, respectively.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAA4
• Uniprot:
  • P31532()