Serum Amyloid A1 Antibody (mc1)

Immunohistochemistry-Paraffin: Serum Amyloid A1 Antibody (mc1) [NB100-65715] - Staining of formalin fixed, paraffin processed human kidney with amyloidosis.

Product Discontinued

Serum Amyloid A1 Antibody (mc1) Summary

• Immunogen: Purified human amyloid A coupled to HRP and kininogen
• Specificity: Recognises human amyloid A deposits and can help to distinguish the AA type from the AL and B2M types of amyloid.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SAA1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen Neat
  • Immunohistochemistry-Paraffin Neat
• Application Notes: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Control:

  Serum Amyloid A1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.5% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Serum Amyloid A1 Antibody (mc1)

• MGC111216
• PIG4
• SAA
• SAA1
• SAA2
• serum amyloid A protein
• Serum Amyloid A1
• TP53I4
• tumor protein p53 inducible protein 4

Background

The serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1 and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrhepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed it's importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP. SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a "housekeeping" role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid arthritis. Amyloidosis is a heterogeneous group of disorders characterized by extracellular deposition of abnormal protein fibrils, which are derived from different proteins. Almost all patients with amyloidosis have some level of renal disorders. The immunoperoxidase technique has the advantage of being able to detect amyloid in formalin-fixed paraffin embedded tissues and is a very specific method for identifying amyloid fibril proteins. Small and minute amounts of amyloid can be detected with both Amyloid P (ab688) and Amyloid A antibodies, and thus could allow earlier treatment before organ damage has occurred.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAA1
• Entrez:
  • 6288(Human)
• Uniprot:
  • P02735()