SERCA2 ATPase Overexpression Lysate

Product Discontinued

SERCA2 ATPase Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_733765. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens SERCA2 ATPase, mRNA.
• Gene: ATP2A2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 114.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SERCA2 ATPase Overexpression Lysate

• ATP2A2
• ATP2B
• ATP2BFLJ20293
• ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2
• ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Calcium pump 2
• Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletalmuscle isoform
• cardiac Ca2+ ATPase
• DAR
• DD
• EC 3.6.3
• EC 3.6.3.8
• Endoplasmic reticulum class 1/2 Ca(2+) ATPase
• FLJ38063
• MGC45367
• sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• SERCA2 ATPase
• SERCA2
• SERCA2DKFZp686P0211
• SR Ca(2+)-ATPase 2

Background

Sarcoplasmic/endoplasmic reticulum Calcium-ATPase 2 (SERCA2) is a magnesium-dependent pump that catalyzes the hydrolysis of ATP and translocates calcium from the cytosol to the sarcoplasmic reticulum lumen. There are 2 SERCA2 isoforms that differ in the 3' UTR. The SERCA2A isoform is highly expressed in heart and slow twitch skeletal muscle and is important to the regulation of the contraction and relaxation cycle, while the SERCA2B isoform is expressed in smooth muscle and adult epidermis. SERCA2 is also known as ATP2A2, ATP2B, DAR, DD, and MGC45367.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ATP2A2