SERCA2 ATPase Antibody (JM10-20)

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (JM10-20) [NBP2-67133] - Staining SERCA2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (JM10-20) [NBP2-67133] - Analysis of paraffin-embedded mouse skin tissue using anti-SERCA2 antibody. Counter stained with hematoxylin.

Flow Cytometry: SERCA2 ATPase Antibody (JM10-20) [NBP2-67133] - Flow cytometric analysis of SERCA2 ATPase was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (JM10-20) [NBP2-67133] - Staining SERCA2 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (JM10-20) [NBP2-67133] - Staining SERCA2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (JM10-20) [NBP2-67133] - Analysis of paraffin-embedded human kidney tissue using anti-SERCA2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (JM10-20) [NBP2-67133] - Analysis of paraffin-embedded human liver cancer tissue using anti-SERCA2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (JM10-20) [NBP2-67133] - Analysis of paraffin-embedded human tonsil tissue using anti-SERCA2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (JM10-20) [NBP2-67133] - Analysis of paraffin-embedded mouse heart tissue using anti-SERCA2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (JM10-20) [NBP2-67133] - Analysis of paraffin-embedded mouse kidney tissue using anti-SERCA2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (JM10-20) [NBP2-67133] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-SERCA2 antibody. Counter stained with hematoxylin.

Product Discontinued

SERCA2 ATPase Antibody (JM10-20) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SERCA2 ATPase aa 999-1,042 / 1,042. (SwissProt: P16615 Human; SwissProt: O55143 Mouse; SwissProt: P11507 Rat)
• Localization: Endoplasmic reticulum membrane, Sarcoplasmic reticulum membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATP2A2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SERCA2 ATPase Antibody (JM10-20)

• ATP2A2
• ATP2B
• ATP2BFLJ20293
• ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2
• ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Calcium pump 2
• Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletalmuscle isoform
• cardiac Ca2+ ATPase
• DAR
• DD
• EC 3.6.3
• EC 3.6.3.8
• Endoplasmic reticulum class 1/2 Ca(2+) ATPase
• FLJ38063
• MGC45367
• sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• SERCA2 ATPase
• SERCA2
• SERCA2DKFZp686P0211
• SR Ca(2+)-ATPase 2

Background

Sarcoplasmic/endoplasmic reticulum Calcium-ATPase 2 (SERCA2) is a magnesium-dependent pump that catalyzes the hydrolysis of ATP and translocates calcium from the cytosol to the sarcoplasmic reticulum lumen. There are 2 SERCA2 isoforms that differ in the 3' UTR. The SERCA2A isoform is highly expressed in heart and slow twitch skeletal muscle and is important to the regulation of the contraction and relaxation cycle, while the SERCA2B isoform is expressed in smooth muscle and adult epidermis. SERCA2 is also known as ATP2A2, ATP2B, DAR, DD, and MGC45367.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP2A2