SERCA2 ATPase Antibody (IID8) [Alexa Fluor™ Plus 555]

• Reactivity: Hu, Mu, Rt, Po, Am, Bv, Ca, Gp, Ha, Pm, Rb, Sh, V-Vi
• Applications: WB, ELISA, Flow, ICC/IF, IHC, B/N
• Clone: IID8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 555

SERCA2 ATPase Antibody (IID8) [Alexa Fluor™ Plus 555] Summary

• Immunogen: Purified canine cardiac sarcoplasmic reticulum.
• Specificity: Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 2 (SERCA2) ATPase. This does not discriminate between the two isoforms.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP2A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SERCA2 ATPase Antibody (IID8) [Alexa Fluor™ Plus 555]

• ATP2A2
• ATP2B
• ATP2BFLJ20293
• ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2
• ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Calcium pump 2
• Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletalmuscle isoform
• cardiac Ca2+ ATPase
• DAR
• DD
• EC 3.6.3
• EC 3.6.3.8
• Endoplasmic reticulum class 1/2 Ca(2+) ATPase
• FLJ38063
• MGC45367
• sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• SERCA2 ATPase
• SERCA2
• SERCA2DKFZp686P0211
• SR Ca(2+)-ATPase 2

Background

Sarcoplasmic/endoplasmic reticulum Calcium-ATPase 2 (SERCA2) is a magnesium-dependent pump that catalyzes the hydrolysis of ATP and translocates calcium from the cytosol to the sarcoplasmic reticulum lumen. There are 2 SERCA2 isoforms that differ in the 3' UTR. The SERCA2A isoform is highly expressed in heart and slow twitch skeletal muscle and is important to the regulation of the contraction and relaxation cycle, while the SERCA2B isoform is expressed in smooth muscle and adult epidermis. SERCA2 is also known as ATP2A2, ATP2B, DAR, DD, and MGC45367.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP2A2