Septin-4 Antibody - BSA Free

Western Blot: Septin-4 Antibody [NBP1-76667] - Human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with Septin-4 antibody at 2 ug /ml.

Immunohistochemistry: Septin-4 Antibody - BSA Free [NBP1-76667] - Immunohistochemistry of Septin-4 in human lung tissue with Septin-4 antibody at 2 u/mL.

Immunocytochemistry/ Immunofluorescence: Septin-4 Antibody - BSA Free [NBP1-76667] - Immunofluorescence of Septin-4 in Human Lung cells with Septin-4 antibody at 20 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Septin-4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Septin-4 Antibody - BSA Free (NBP1-76667) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human ARTS. The immunogen is located within the first 50 amino acids of ARTS. Amino Acid Squence: KRFLEDTTDDGE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEPTIN4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 2 ug/ml
• Application Notes: A band at 32 kDa can be detected in Western Blot.
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Septin-4 Antibody - BSA Free

• Apoptosis-related protein in the TGF-beta signaling pathway
• ARTSPeanut-like protein 2
• Bradeion beta
• BRADEION
• Brain protein H5
• CE5B3
• Cell division control-related protein 2
• Cerebral protein 7
• H5
• hCDCREL-2SEP4
• hucep-7
• MART
• peanut-like 2 (Drosophila)
• peanut-like 2
• PNUTL2CE5B3 beta
• septin 4
• septin-4
• septin-M

Background

Apoptosis is related to many diseases and development. Mitochondrial proteins, such as cytochrome c, Apaf-1, and AIF play important role in apoptosis. A novel mitochondrial septin-like protein was identified recently and designated ARTS for apoptosis related protein in TGF-b signaling pathway. ARTS that is encoded by the human septin H5/PNUTL2/CDCrel2b gene is located to mitochondria and translocates to the nucleus when apoptosis occurs. ARTS is expressed in many tissues. It enhances cell death induced by TGF-b and, to a lesser extent, by other apoptotic agents, such as TNF-a and Fas ligand.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEPTIN4
• Uniprot:
  • AAG45673(Human)