GPR50 Antibody - Azide and BSA Free

Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 competes with FKBP12 for the binding to TBRI. Competition between FKBP12 and GPR50 for TBRI binding was checked by precipitating FKBP12 from total brain and lung lysates of wt and GPR50ko mice and revealed with anti-TVRI. Total lysate was addressed for expression of FKBP12, TBRI, and GPR50 with corresponding antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.

Flow Cytometry: GPR50 Antibody [H00009248-B01P] - Analysis of negative control 293 cells (Black) and GPR50 expressing 293 cells (Green) using GPR50 purified mouse polyclonal antibody.

Western Blot: GPR50 Antibody [H00009248-B01P] - Analysis of GPR50 expression in transfected 293T cell line by GPR50 polyclonal antibody. Lane 1: GPR50 transfected lysate(67.87 KDa). Lane 2: Non-transfected lysate.

Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 promotes ligand-independent activation of TBRI signaling. Detection of p-Smad3, p-Stat-3, p-Jak2 (c) and p-Smad2 (d) in lysates of hypothalamus and cortex of wt and GPR50 ko mice. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.

Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 competes with FKBP12 for the binding to TBRI. Competition between FKBP12 and GPR50 for TBRI binding was checked by precipitating FKBP12 from total brain and lung lysates of wt and GPR50ko mice and revealed with anti-TBRI. Total lysate was addressed for expression of FKBP12, TBRI, and GPR50 with corresponding antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.

Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 phosphorylate & activate T beta RI independently of T beta RII. a SNU638 cells were transfected with the indicated plasmids, stimulated for 1 h with TGF beta (2 ng/mL) & pretreated or not overnight with SB431542 at 10 µM. p-Smad3 & total Smad3 levels & expression of transfected plasmids were determined by western blot in cell lysates. b Co-immunoprecipitation of GRP50 & T beta RI in lysates of SNU638 cells expressing HA-T beta RI alone or together with GPR50 delta 4. Total lysates were used as expression control (c) SNU638 cells were transfected with the indicated plasmids, stimulated TGF beta (2 ng/mL, 1 h). Total T beta RI was immunoprecipitated with anti-Flag antibody & immunoblotted for anti-T beta RIp-S165. Below, the same blot was immunoblotted with anti-Flag to show the amount of T beta RI precipitation in cell lysates. d Phospho-Smad3 & Smad2/3 levels were determined in lysates of SNU638 cells stimulated or not with TGF beta (2 ng/mL, 1 h) & expressing the indicated proteins (as verified by western blot). The myc-MT2 melatonin receptor & the myc-MT2-GPR50Cter served as negative controls. Representative results are shown for all the panels. Similar results were obtained in at least two additional experiments. See also Supplementary Fig. 4 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 phosphorylate & activate T beta RI independently of T beta RII. a SNU638 cells were transfected with the indicated plasmids, stimulated for 1 h with TGF beta (2 ng/mL) & pretreated or not overnight with SB431542 at 10 µM. p-Smad3 & total Smad3 levels & expression of transfected plasmids were determined by western blot in cell lysates. b Co-immunoprecipitation of GRP50 & T beta RI in lysates of SNU638 cells expressing HA-T beta RI alone or together with GPR50 delta 4. Total lysates were used as expression control (c) SNU638 cells were transfected with the indicated plasmids, stimulated TGF beta (2 ng/mL, 1 h). Total T beta RI was immunoprecipitated with anti-Flag antibody & immunoblotted for anti-T beta RIp-S165. Below, the same blot was immunoblotted with anti-Flag to show the amount of T beta RI precipitation in cell lysates. d Phospho-Smad3 & Smad2/3 levels were determined in lysates of SNU638 cells stimulated or not with TGF beta (2 ng/mL, 1 h) & expressing the indicated proteins (as verified by western blot). The myc-MT2 melatonin receptor & the myc-MT2-GPR50Cter served as negative controls. Representative results are shown for all the panels. Similar results were obtained in at least two additional experiments. See also Supplementary Fig. 4 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, KO
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GPR50 Antibody - Azide and BSA Free Summary

• Immunogen: GPR50 (AAI03697.1, 1 a.a. - 617 a.a.) full-length human protein. MGPTLAVPTPYGCIGCKLPQPEYPPALIIFMFCAMVITIVVDLIGNSMVILAVTKNKKLRNSGNIFVVSLSVADMLVAIYPYPLMLHAMSIGGWDLSQLQCQMVGFITGLSVVGSIFNIVAIAINRYCYICHSLQYERIFSVRNTCIYLVITWIMTVLAVLPNMYIGTIEYDPRTYTCIFNYLNNPVFTVTIVCIHFVLPLLIVGFCYVRIWTKVLAARDPAGQNPDNQLAEVRNFLTMFVIFLLFAVCWCPINVLTVLVAVSPKEMAGKIPNWLYLAAYFIAYFNSCLNAVIYGLLNENFRREYWTIFHAMRHPIIFFSGLISDIREMQEARTLARARAHARDQAREQDRAHACPAVEETPMNVRNVPLPGDAAAGHPDRASGHPKPHSRSSSAYRKSASTHHKSVFSHSKAASGHLKPVSGHSKPASGHPKSATVYPKPASVHFKADSVHFKGDSVHFKPDSVHFKPASSNPKPITGHHVSAGSHSKSAFSAATSHPKPTTGHIKPATSHAEPTTADYPKPATTSHPKPTAADNPELSASHCPEIPAIAHPVSDDSDLPESASSPAAGPTKPAASQLESDTIADLPDPTVVTTSTNDYHDVVVVDVEDDPDEMAV
• Specificity: Reacts with G protein-coupled receptor 50.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: GPR50
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Knockout Validated
  • Western Blot 1:500
• Application Notes: This antibody is reactive against transfected lysate in western blot, FACS analysis, and as a detection antibody in ELISA. It is also useful in FACS analysis.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:29572483).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GPR50 Antibody - Azide and BSA Free

• G protein-coupled receptor 50H9Mel1c
• GPR50
• H9
• melatonin-related receptor
• MGC125342
• MTR1L

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR50 Antibody (H00009248-B01P)(1)

Publication using H00009248-B01P

• Wojciech S, Ahmad R, Belaid-Choucair Z et al. The orphan GPR50 receptor promotes constitutive TGF beta receptor signaling and protects against cancer development Nat Commun 2018-03-23 [PMID: 29572483] (WB, Rat)

Bioinformatics

• Gene Symbol: GPR50
• Entrez:
  • 9248(Human)
• Uniprot:
  • AAI03697.1(Human)