Septin-2 Antibody

Western Blot: Septin-2 Antibody [NBP1-85212] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Western Blot: Septin-2 Antibody [NBP1-85212] - Stable septin-2 knockdown shows a decrease in proliferation. Verification of septin-2 knockdown at protein level visualized by Western blot. A single blot was probed simultaneously for septin-2 and GAPDH as a loading control. The original blot can be seen in Supplementary Figure 4. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.26836), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Septin-2 Antibody [NBP1-85212] - Imaging of cultured rat corrtical astrocytes. Image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human testis shows strong membranous/cytoplasmic positivity in cells in seminiferous ducts.

Western Blot: Septin-2 Antibody [NBP1-85212] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/Immunofluorescence: Septin-2 Antibody [NBP1-85212] - Staining of human cell line U-2 OS shows localization to nucleus, nucleoli & actin filaments. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human small intestine shows weak cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Septin-2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: WGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEPT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: IHC, WB reported in scientific literature (PMID: 25293760). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Septin-2 antibody validated for ICC/IF from a verified customer review.
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Septin-2 Recombinant Protein Antigen (NBP1-85212PEP)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25293760).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Septin-2 Antibody

• DIFF6NEDD-5
• hNedd5
• KIAA0158Neural precursor cell expressed developmentally down-regulated protein 5
• NEDD5Pnutl3
• neural precursor cell expressed, developmentally down-regulated 5
• septin 2
• septin-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Septin-2 Antibody (NBP1-85212)(3)

Publications using NBP1-85212

• Lim RR, Hainsworth DP, Mohan RR, Chaurasia SS Septin-2 is overexpressed in epithelial ovarian cancer and mediates proliferation via regulation of cellular metabolic proteins Oncotarget 2019-04-26 [PMID: 31105878] (KD, WB, Human)
• Volceanov L, Herbst K, Biniossek M et al. Septins Arrange F-Actin-Containing Fibers on the Chlamydia trachomatis Inclusion and Are Required for Normal Release of the Inclusion by Extrusion. mBio 2014-10-07 [PMID: 25293760] (IF/IHC, WB, Human)
• Froidevaux-Klipfel L, Poirier F, Boursier C et al. Modulation of septin and molecular motor recruitment in the microtubule environment of the Taxol-resistant human breast cancer cell line MDA-MB-231. Proteomics 2011-10-01 [PMID: 21761557]

Review for Septin-2 Antibody (NBP1-85212) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer ICC Rat 06/27/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Rat cortical astrocytes
• Species: Rat

Comments

• Comments: Fixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT
  
  Blocking Solution & Duration: 1% BSA in PBS with 0.1% triton, 1 hour RT
  
  Primary Antibody Diluent and Dilutions Tested: 1:1000 in 1% BSA in PBS, over night 4°C
  
  Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-rabbit Alexa647, 1:2000 in PBS, 2 hours, RT

Bioinformatics

• Gene Symbol: SEPT2
• Entrez:
  • 4735(Human)
• Uniprot:
  • Q15019(Human)