Septin-2 Antibody

Images

 
Western Blot: Septin-2 Antibody [NBP1-85212] - Stable septin-2 knockdown shows a decrease in proliferation. Verification of septin-2 knockdown at protein level visualized by Western blot. A single blot was probed ...read more
Immunocytochemistry/ Immunofluorescence: Septin-2 Antibody [NBP1-85212] - Imaging of cultured rat corrtical astrocytes. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human testis shows strong membranous/cytoplasmic positivity in cells in seminiferous ducts.
Western Blot: Septin-2 Antibody [NBP1-85212] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Genetic Strategies: Western Blot: Septin-2 Antibody [NBP1-85212] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. ...read more
Immunocytochemistry/ Immunofluorescence: Septin-2 Antibody [NBP1-85212] - Staining of human cell line U-2 OS shows localization to nucleus, nucleoli & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human small intestine shows weak cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Septin-2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: WGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGH
Specificity
Specificity of human Septin-2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEPT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Knockdown Validated
Application Notes
IHC, WB reported in scientific literature (PMID: 25293760). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Septin-2 Recombinant Protein Antigen (NBP1-85212PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-85212 in the following applications:

Publications
Read Publications using
NBP1-85212 in the following applications:

  • IHC
    1 publication
  • KD
    1 publication
  • WB
    2 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25293760).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Septin-2 Antibody

  • DIFF6NEDD-5
  • hNedd5
  • KIAA0158Neural precursor cell expressed developmentally down-regulated protein 5
  • NEDD5Pnutl3
  • neural precursor cell expressed, developmentally down-regulated 5
  • septin 2
  • septin-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD

Publications for Septin-2 Antibody (NBP1-85212)(3)

Review for Septin-2 Antibody (NBP1-85212) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP1-85212:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Septin-2 NBP1-85212
Enlarge
5
reviewed by:
Janosch Heller
ICC Rat 06/27/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedRat cortical astrocytes
SpeciesRat

Comments

CommentsFixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT

Blocking Solution & Duration: 1% BSA in PBS with 0.1% triton, 1 hour RT

Primary Antibody Diluent and Dilutions Tested: 1:1000 in 1% BSA in PBS, over night 4°C

Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-rabbit Alexa647, 1:2000 in PBS, 2 hours, RT

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Septin-2 Antibody (NBP1-85212) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Septin-2 Products

Bioinformatics Tool for Septin-2 Antibody (NBP1-85212)

Discover related pathways, diseases and genes to Septin-2 Antibody (NBP1-85212). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Septin-2 Antibody (NBP1-85212)

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PTMs for Septin-2 Antibody (NBP1-85212)

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Research Areas for Septin-2 Antibody (NBP1-85212)

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Blogs on Septin-2

There are no specific blogs for Septin-2, but you can read our latest blog posts.
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Contact Information

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Recent Reviews

5
5
1
4
0
3
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2
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1
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Janosch Heller
06/27/2017
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol SEPT2
Entrez
Uniprot