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![Western Blot: Septin-2 Antibody [NBP1-85212] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Western-Blot-NBP1-85212-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Septin-2 Antibody [NBP1-85212] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Western Blot: Septin-2 Antibody [NBP1-85212] - Stable septin-2 knockdown shows a decrease in proliferation. Verification of septin-2 knockdown at protein level visualized by Western blot. A single blot was probed simultaneously for septin-2 and GAPDH as a loading control. The original blot can be seen in Supplementary Figure 4. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.26836), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Western-Blot-NBP1-85212-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-2 Antibody [NBP1-85212] - Imaging of cultured rat corrtical astrocytes. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85212-img0012.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human testis shows strong membranous/cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85212-img0018.jpg)
![Western Blot: Septin-2 Antibody [NBP1-85212] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Western-Blot-NBP1-85212-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-2 Antibody [NBP1-85212] - Staining of human cell line U-2 OS shows localization to nucleus, nucleoli & actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85212-img0011.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85212-img0015.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85212-img0016.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human small intestine shows weak cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85212-img0017.jpg)
![Western Blot: Septin-2 Antibody [NBP1-06076] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.05 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Western-Blot-NBP1-06076-img0002.jpg "Western Blot: Septin-2 Antibody [NBP1-06076] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.05 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Cortex. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0005.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Breast tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0003.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Tonsil tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0004.jpg)
![Immunohistochemistry: Septin-2 Antibody (8H4) [NBP3-43032] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (Septin-2 Antibody) at dilution of 1:800](http://images.novusbio.com/fullsize/nbp3-43032_mouse-septin-2-mab-8h4-101220241715429.jpg "Immunohistochemistry: Septin-2 Antibody (8H4) [NBP3-43032] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (Septin-2 Antibody) at dilution of 1:800")
![Immunocytochemistry/ Immunofluorescence: Septin-2 Antibody (8H4) [NBP3-43032] - Immunofluorescence analysis of U2OS cells using Septin-2 antibody (FNab07722). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-43032_mouse-septin-2-mab-8h4-101220241723695.jpg)
![Western Blot: Septin-2 Antibody (8H4) [NBP3-43032] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (Septin-2 Antibody) at 1:8000.](http://images.novusbio.com/fullsize/nbp3-43032_mouse-septin-2-mab-8h4-101220241734110.jpg)
![Western Blot: Septin-2 Antibody [NBP1-31496] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Western-Blot-NBP1-31496-img0004.jpg "Western Blot: Septin-2 Antibody [NBP1-31496] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE, antibody diluted at 1:2000.")
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-31496] - Sample: Paraffin-embedded mouse ovary. Septin-2 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-31496-img0005.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-31496] - Paraffin-embedded H441 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-31496-img0003.jpg)
![Western Blot: Septin-2 Antibody [NBP2-20301] - Sample (30 ug of whole cell lysate) A: U87-MG B: SK-N-SH 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Western-Blot-NBP2-20301-img0001.jpg "Western Blot: Septin-2 Antibody [NBP2-20301] - Sample (30 ug of whole cell lysate) A: U87-MG B: SK-N-SH 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP2-20301] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-20301-img0002.jpg)
![Immunoprecipitation:Septin-2 AntibodyNBP3-30511] -Detection of human Septin 2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Septin 2 antibody NBP3-30511 (lot NBP3-30511-1) used for IP at 6 µg/mg lysate. Septin 2 was also immunoprecipitated by rabbit anti-Septin 2.. For blotting immunoprecipitated Septin 2, NBP3-30511 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-30511_rabbit-septin-2-pab-3012202412262521.jpg "Immunoprecipitation:Septin-2 AntibodyNBP3-30511] -Detection of human Septin 2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Septin 2 antibody NBP3-30511 (lot NBP3-30511-1) used for IP at 6 µg/mg lysate. Septin 2 was also immunoprecipitated by rabbit anti-Septin 2.. For blotting immunoprecipitated Septin 2, NBP3-30511 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunoprecipitation:[NBP3-30591]-Detection of human Septin 2 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified rabbit anti-Septin 2 antibody used for IP at 6 µg/mg lysate. Septin 2 was also immunoprecipitated by rabbit anti-Septin 2 antibody. For blotting immunoprecipitated Septin 2, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-30591_rabbit-septin-2-pab-2312025128864.jpg "Immunoprecipitation:[NBP3-30591]-Detection of human Septin 2 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified rabbit anti-Septin 2 antibody used for IP at 6 µg/mg lysate. Septin 2 was also immunoprecipitated by rabbit anti-Septin 2 antibody. For blotting immunoprecipitated Septin 2, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Western Blot: Septin-2 Antibody (9N1B3) [NBP3-16653] - Western blot analysis of extracts of various cell lines, using Septin-2 Rabbit mAb (NBP3-16653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Septin-2-Antibody-9N1B3-Western-Blot-NBP3-16653-img0001.jpg "Western Blot: Septin-2 Antibody (9N1B3) [NBP3-16653] - Western blot analysis of extracts of various cell lines, using Septin-2 Rabbit mAb (NBP3-16653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Septin-2 Antibody [NBP1-58217] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Western-Blot-NBP1-58217-img0003.jpg "Western Blot: Septin-2 Antibody [NBP1-58217] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.")
![Western Blot: Septin-2 Overexpression Lysate (Adult Normal) [NBL1-07140] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-2.](http://images.novusbio.com/fullsize/Septin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07140-img0002.jpg "Western Blot: Septin-2 Overexpression Lysate (Adult Normal) [NBL1-07140] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-2.")
![Western Blot: Septin-2 Overexpression Lysate (Adult Normal) [NBP2-10989] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-2.](http://images.novusbio.com/fullsize/Septin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10989-img0001.jpg "Western Blot: Septin-2 Overexpression Lysate (Adult Normal) [NBP2-10989] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-2.")