Semaphorin 6C Antibody (254506) [Alexa Fluor® 488] Summary
Immunogen |
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6C Ala25-Val601 Accession # NP_112175 |
Specificity |
Detects human Semaphorin 6C in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 6A, 6B, 6D, 7A, recombinant mouse Semaphorin 3C, 3E, 3F, or 6C is observed. |
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Mouse |
Purity Statement |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied |
Buffer |
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Semaphorin 6C Antibody (254506) [Alexa Fluor® 488]
Background
Semaphorin 6C (Sema6C; previously Sema Y) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1 ‑ 3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that exhibit neuropilin-independent binding to specific plexin A receptors (1 ‑ 3). Sema6C is expressed developmentally in subregions of the central and peripheral nervous systems, heart, and kidney, and primarily in skeletal muscle in adults (3, 4). Human Sema6C cDNA encodes 930 aa, including a 24 aa signal sequence, a 579 aa extracellular domain (ECD) including the Sema domain, a 21 aa transmembrane sequence and a 306 aa cytoplasmic portion. Alternate exon splicing creates a 922 aa short form (Sema6C.3) that is lacking aa 184 - 223 within the Sema domain, but contains 32 unique aa inserted after aa 586; postnatally, this form predominates in muscle (2, 3). A 962 aa form contains only the insert. The 930 aa “long form” predominates in brain, especially in areas of increased plasticity (4). Human Sema6C ECD shares 92%, 93%, 94%, 94%, 95% and 87% aa identity with corresponding mouse, rat, porcine, bovine, equine and canine sequences, respectively. Sema6C, along with Sema6D, is co-expressed with and binds to Plexin A1 (5). This interaction is thought to guide proprioceptive peripheral neurons by repulsion, excluding them from the superficial dorsal horn of the spinal cord (5). Sema6C is down‑regulated and redistributed following denervation or axotomy, potentially promoting regrowth (4, 6). In muscle, Sema6C is concentrated at neuromuscular junctions (6).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: ICC, IHC, WB
Species: Mu
Applications: IHC, WB
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
Species: Hu, Rt
Applications: ICC/IF, IHC-P, WB
Species: Mu
Applications: IHC, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Species: Hu
Applications: ICC/IF
Species: Hu
Applications: ICC/IF, WB
Species: Hu, Mu
Applications: WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Mu, Rt
Applications: Block, CyTOF-ready, Flow, IHC, WB
Species: Hu
Applications: CyTOF-ready, Flow, Simple Western, WB
Species: Hu
Applications: IP, WB
Species: Hu
Applications: BA
Species: Hu, Mu, Rt
Applications: ICC, WB
Species: Hu
Applications: Simple Western, WB
Species: Hu, Mu, Rt
Applications: Simple Western, WB
Species: Hu, Mu
Applications: ICC/IF, KD, WB
Species: Hu
Applications: WB
Publications for Semaphorin 6C Antibody (FAB2219G) (0)
There are no publications for Semaphorin 6C Antibody (FAB2219G).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Semaphorin 6C Antibody (FAB2219G) (0)
There are no reviews for Semaphorin 6C Antibody (FAB2219G).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Semaphorin 6C Antibody (FAB2219G) (0)
Secondary Antibodies
| |
Isotype Controls
|
Additional Semaphorin 6C Products
Blogs on Semaphorin 6C