Semaphorin 5A Antibody

Immunohistochemistry-Paraffin: Semaphorin 5A Antibody [NB100-41107] - (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Semaphorin 5A Antibody Summary

• Immunogen: Peptide with sequence DEATKKACYSKGKS corresponding to internal region according to NP_003957.1.
• Specificity: The immunizing peptide corresponds to a part of the extracellular domain.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SEMA5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Preliminary experiments gave an approx. 90 kDa band in mouse brain lysates and approx. 25 kDa band in human brain (cerebellum), colon, heart and kidney lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121 kDa band according to NP_003957.1. The 90 kDa band and 25 kDa band were successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows membanous and vesicular staining in PCT cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Semaphorin 5A Antibody

• FLJ12815
• sema domain, seven thrombospondin repeats (type 1 and type 1-like)
• Sema F
• SEMA5A
• SEMAF
• Semaphorin 5A
• semaphorin F
• semaphorin-5A
• Semaphorin-F
• semF
• transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A

Background

Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function. The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively. The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 5A Antibody (NB100-41107)(1)

Publication using NB100-41107

• Maraganore DM, de Andrade M, Lesnick TG et al. High-resolution whole-genome association study of Parkinson disease Am J Hum Genet 2005-11-01 [PMID: 16252231]

Bioinformatics

• Gene Symbol: SEMA5A
• Entrez:
  • 9037(Human)
  • 20356(Mouse)
  • 310207(Rat)
• Uniprot:
  • NP_003957.1(Human)